Conduction of a chemical structure-guided metabolic phenotype analysis method targeting phenylpropane pathway via LC-MS: Ginkgo biloba and soybean as examples
Conduction of a chemical structure-guided metabolic phenotype analysis method targeting phenylpropane pathway via LC-MS: Ginkgo biloba and soybean as examples
复制标题
DOI：
10.1016/j.foodchem.2022.133155
复制
发表时间：
2022-05-13
期刊：
影响因子：
8.8
通讯作者：
Zhou, Gongke
中科院分区：
文献类型：
--
作者：
Meng, Jie;Zhang, Yiran;Zhou, Gongke
苯丙烷途径（PPP）是研究最广泛的代谢途径之一。这条途径生物合成许多重要的活性成分，如苯丙素和黄酮类化合物，影响食品的风味，味道和营养。如何阐明PPP的代谢表型是食品研究和开发的基础。在这项研究中，我们设计了一个结构周期表充满了103个代谢产物从PPP。采用液相色谱-串联三重四极杆质谱（LC-MS）多反应模式，以高分辨率和高灵敏度对所有化合物，尤其是62种结构异构体进行定性和定量。以银杏和大豆为试材，对该方法进行了实际应用研究：首次阐明了银杏叶片中PPP的时空代谢平衡，首次证实了盐胁迫和干旱胁迫可以改变大豆叶片中PPP的生物合成趋势，使其产生更多的异黄酮。
The phenylpropane pathway (PPP) is one of the most extensively investigated metabolic routes. This pathway biosynthesizes many important active ingredients such as phenylpropanoids and flavonoids that affect the flavor, taste and nutrients of food. How to elucidate the metabolic phenotype of PPP is fundamental in food research and development. In this study, we designed a structural periodical table filled with 103 metabolites produced from PPP. All of them especially the 62 structural isomers were qualified and quantified with high resolution and sensitivity via multiple reaction mode in liquid chromatography tandem triple quadrupole mass spectrometry. Ginkgo biloba and soybean were used as samples for the practical application of this method: The delicate spatial-temporal metabolic balance of PPP from ginkgo biloba has been first elucidated; It is first confirmed that the salt and draught stresses could redirect the biosynthesis trend of PPP to produce more isoflavones in soybean leaves.