Intrauterine Growth Restriction Impairs Small Intestinal Mucosal Immunity in Neonatal Piglets
Intrauterine Growth Restriction Impairs Small Intestinal Mucosal Immunity in Neonatal Piglets
复制标题
宫内生长受限损害新生仔猪小肠粘膜免疫
DOI：
10.1369/0022155414532655
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发表时间：
2014-07-01
影响因子：
3.2
通讯作者：
Wang, Tian
中科院分区：
文献类型：
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作者：
Dong, Li;Zhong, Xiang;Wang, Tian
宫内生长受限(IUGR)在仔猪和人类新生儿群体中都是一个非常常见的问题。我们假设IUGR新生儿从出生起就有肠道粘膜免疫受损。以新生仔猪为IUGR模型，观察免疫器官重量、小肠重量和长度、肠道形态、上皮内免疫细胞数、细胞因子和免疫球蛋白水平以及细胞因子的相关基因表达。IUGR新生仔猪的免疫器官绝对重量和SI长度较低，胸腺、脾和肠系膜淋巴结的相对重量较小，SIS较薄但较长。IUGR新生仔猪SIS的绒毛受损、锯齿状，微绒毛变短，自噬小体存在，线粒体肿胀，绒毛表面积减少。在IUGR新生儿的SIS中，我们还发现了少量的上皮杯状细胞和淋巴细胞。此外，我们还检测到胎儿宫内发育迟缓新生儿细胞因子肿瘤坏死因子-α和干扰素-γ水平的降低以及细胞因子基因表达的降低。综上所述，IUGR对新生仔猪SI的黏膜免疫功能有损害作用，回肠是损伤的主要部位。
Intrauterine growth restriction (IUGR) is a very common problem in both piglet and human neonate populations. We hypothesized that IUGR neonates have impaired intestinal mucosal immunity from birth. Using neonatal piglets as IUGR models, immune organ weights, the weight and length of the small intestine (SI), intestinal morphology, intraepithelial immune cell numbers, levels of cytokines and immunoglobulins, and the relative gene expression of cytokines in the SI were investigated. IUGR neonatal piglets were observed to have lower absolute immune organ weight and SI length, decreased relative weights of the thymus, spleen, mesenteric lymph node, and thinner but longer SIs. Damaged and jagged villi, shorter microvilli, presence of autophagosomes, swelled mitochondria, and decreased villus surface areas were also found in the SIs of IUGR neonatal piglets. We also found a smaller number of epithelial goblet cells and lymphocytes in the SIs of IUGR neonates. In addition, we detected reduced levels of the cytokines TNF-α and IFN-γ and decreased gene expression of cytokines in IUGR neonates. In conclusion, IUGR was shown to impair the mucosal immunity of the SI in neonatal piglets, and the ileum was the major site of impairment.