Vesicle size, size distribution, stability, and rheological properties of liposomes coated with water-soluble chitosans of different molecular weights and concentrations
Vesicle size, size distribution, stability, and rheological properties of liposomes coated with water-soluble chitosans of different molecular weights and concentrations
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DOI：
10.1081/lpr-100108463
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发表时间：
2001-01-01
影响因子：
4.4
通讯作者：
Fang, HJ
中科院分区：
文献类型：
--
作者：
Chen, RH;Win, HP;Fang, HJ
本研究的目的是探讨不同分子量和浓度的水溶性壳聚糖包衣对脂质体囊泡大小、粒径分布、稳定性和表观粘度的影响。结果表明，不同浓度、不同分子量的水溶性壳聚糖包覆的脂质体囊泡尺寸随微流化次数的增加而减小，然后趋于恒定。脂质体进行相同的微流化处理较大的那些涂覆有较高浓度（相同分子量的壳聚糖）或涂覆有较低分子量（在相同浓度）的水溶性壳聚糖。不同分子量水溶性壳聚糖包覆脂质体的平均粒径随微流化处理次数的增加而减小。水溶性壳聚糖包覆脂质体的表观粘度在第一次微流化处理后下降，第三次微流化处理后趋于稳定。不同处理次数的脂质体的表观粘度随壳聚糖浓度和分子量的增加而增大，不同分子量的壳聚糖浓度为0.5%时，脂质体表现为假塑性流体。在相同剪切速率下，微流化次数多的脂质体的表观粘度大于微流化次数少的脂质体的表观粘度。包被有水溶性壳聚糖的脂质体比没有包被的脂质体更稳定。
The objectives of this study were to explore the effects of coating with water-soluble chitosans of different molecular weights and concentrations on vesicle size, size distribution, stability, and apparent viscosity of liposomes. The results indicate that the vesicle size of liposomes coated with different concentrations and different molecular weights of water-soluble chitosans decreased with increasing passes of microfluidizing treatment, then reached a constant value. Liposomes subjected to the same microfluidization treatment were larger for those coated with a higher concentration (of the same molecular weight chitosan) or coated with a lower molecular weight (at the same concentration) of water-soluble chitosans. The average particle size of liposomes coated with different molecular weights of water-soluble chitosans decreased with increasing number of passes of microfluidizing treatment. The apparent viscosity of liposomes coated with water-soluble chitosans decreased after the first pass, then reached a constant value after the third pass of microfluidizing treatment. Apparent viscosities of liposomes subjected to different passes of microfluidization treatment were larger for those coated with a higher concentration or a higher molecular weight of water-soluble chitosans, Liposomes coated with 0.5% of different molecular weights of water-soluble chitosans behaved as pseudoplastic fluids. At the same shear rate, the apparent viscosities of liposomes subjected to more passes of microfluidizing treatment were larger than those subjected to fewer passes of microfluidizing treatment. Liposomes coated with water-soluble chitosans are more stable than those without a coating.