THE JAM TEST - A SIMPLE ASSAY FOR DNA FRAGMENTATION AND CELL-DEATH
THE JAM TEST - A SIMPLE ASSAY FOR DNA FRAGMENTATION AND CELL-DEATH
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DOI：
10.1016/0022-1759(91)90325-a
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发表时间：
1991-12-15
影响因子：
2.2
通讯作者：
MATZINGER, P
中科院分区：
文献类型：
--
作者：
MATZINGER, P
目前大多数用于测量细胞死亡的方法是基于质膜解体和随后的细胞质释放。 相关细胞通常装载有标记物（通常为Cr-51或I-125），测量其释放。 我在这里描述了一种方法，基于最近的证据，即垂死细胞经常将其DNA降解成小片段，该方法测量活细胞保留的DNA，而不是垂死细胞丢失的细胞成分。 该测定基本上像当前的细胞裂解测定一样建立，并且像细胞增殖测定一样收获。 它比目前的标准Cr-51释放测定法更快、更灵敏、更容易设置、更便宜和更安全。
Most current methods for measuring cell death are based on plasma membrane disintegration and the consequent release of cytoplasm. The relevant cells are usually loaded with a label (usually Cr-51 or I-125), the release of which is measured. I describe here a method, based on the recent evidence that dying cells often degrade their DNA into small fragments, which measures the DNA retained by living cells rather than the cellular components lost by dying cells. The assay is set up essentially like the current cell lysis assays and harvested like a cell proliferation assay. It is faster, more sensitive, easier to set up, less expensive and safer than the current standard Cr-51 release assay.