The postpartum endometrial inflammatory response: a normal physiological event with potential implications for bovine fertility
The postpartum endometrial inflammatory response: a normal physiological event with potential implications for bovine fertility
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DOI：
10.1071/rd11153
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发表时间：
2012-01-01
影响因子：
1.9
通讯作者：
O'Farrelly, Cliona
中科院分区：
文献类型：
--
作者：
Chapwanya, Aspinas;Meade, Kieran G.;O'Farrelly, Cliona
产犊后，牛子宫内膜发生了明显的形态和功能变化，这对随后的再繁殖是必要的。这些关键事件的监管和整合在很大程度上是不明确的。本研究在产后15、30和60天对13头健康的奶牛（其中10头随后怀孕）进行子宫内膜拭子和活检，以表征先天和炎症基因表达谱。15 DPP时子宫内膜活检显示严重炎症（每高倍视野约75个白细胞），30 DPP时开始消退，60 DPP时完全消退。15 DPP时，所有奶牛的重度炎症均与子宫感染同时发生，CD45、IL8、IL6、IL1、TNF、TAP、SAA3、HP等16种基因的表达均显著高于60 DPP （P < 0.05）。所有这些参数在30 DPP时开始恢复到正常的生理水平。与60 DPP相比，15 DPP时血清IL-8蛋白浓度升高(78 pg mL(-1) vs 48 pg mL(-1))；P = 0.02)。这些结果表明，子宫内膜炎症、白细胞浸润以及促炎、抗菌和急性期蛋白基因的表达增加是产后的预期特征，对细菌清除和子宫复旧至关重要。
After calving, the bovine endometrium undergoes marked morphological and functional changes that are necessary for subsequent re-breeding. Regulation and integration of these key events are largely uncharacterised. Here, endometrial swabs and biopsies were taken at 15, 30 and 60 days postpartum (DPP) from 13 healthy primiparous cows, 10 of which subsequently conceived, with a view to characterising innate and inflammatory gene expression profiles. Endometrial biopsies exhibited severe inflammation (>75 leukocytes per high-power field) at 15 DPP, which had begun to resolve by 30 DPP and had completely resolved by 60 DPP. The severe inflammation at 15 DPP coincided with uterine infection in all cows and a significant increase (P < 0.05) in the expression of all of 16 genes investigated, including CD45, IL8, IL6, IL1, TNF, TAP, SAA3 and HP at 15 DPP, relative to 60 DPP. All of these parameters had begun to return to normal physiological levels at 30 DPP. Systemically, serum protein concentrations of IL-8 were elevated at 15 DPP compared with 60 DPP (78 pg mL(-1) vs 48 pg mL(-1); P = 0.02). These results indicate that endometrial inflammation, leukocyte infiltration and increased expression of pro-inflammatory, antimicrobial and acute-phase protein genes are expected features of the postpartum period, critical to bacterial clearance and uterine involution.