Comparative effects of water‐bath‐ and microwave‐induced hyperthermia on survival of Chinese hamster ovary (CHO) cells
Comparative effects of water‐bath‐ and microwave‐induced hyperthermia on survival of Chinese hamster ovary (CHO) cells
复制标题
水浴和微波诱导热疗对中国仓鼠卵巢（CHO）细胞存活的影响比较
DOI：
10.1029/rs014i06sp00117
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发表时间：
1979
期刊：
影响因子：
1.6
通讯作者：
L. Dethlefsen
中科院分区：
文献类型：
--
作者：
G. Livingston;Curtis C. Johnson;L. Dethlefsen
将处于对数生长期的非稳态CHO细胞悬浮液置于水浴或2450-MHz微波热疗中，温度为44 ℃，持续60分钟。高温处理后，立即将细胞在37°C下平衡，计数，并平板接种进行集落测定，以定量细胞的存活率。通过将长尺寸的塑料培养管（3 ml培养基中含有8×105个细胞）插入平行于电场矢量的波导中进行微波加热;间歇施加CW能量以将悬浮液保持在44±0.1°C。通过将培养管浸入44±0.05°C的水浴中，在来自相同培养物的类似细胞样品中诱导高热。通过用液晶光纤温度探针监测细胞悬浮液，匹配两种热疗的温升曲线。在可比的条件下，两种来源的高温细胞的存活率没有显着差异。存活曲线的Do（指数区域的斜率）对于水浴处理为8.3分钟，而对于微波处理为8.1分钟。类似地，水浴处理的Dq（指示细胞修复的存活曲线的量度）为23.4分钟，微波处理的Dq为23.7分钟。样品内加热的均匀性和准确的测温被认为是非常关键的奇偶校验的演示。
Suspensions of asychronous CHO cells in the log phase of growth were subjected to a water-bath or to 2450-MHz-microwave hyperthermia at a temperature of 44°C for a period of 60 minutes. Immediately following hyperthermic treatment, the cells were equilibrated at 37°C, counted, and plated out for colony assays, to quantitate survival of cells. Microwave heating was performed by inserting the long dimension of a plastic culture tube, which contained 8×105 cells in 3 ml of media, into a waveguide parallel to the E-field vector; CW energy was intermittently applied to maintain the suspension at 44±0.1°C. Hyperthermia was induced in a similar sample of cells from the same culture by immersing the culture tubes into a water bath at 44±0.05°C. Temperature-rise profiles of the two hyperthermic treatments were matched by monitoring suspensions of cells with the Liquid Crystal Fiberoptic temperature probe. Cells subjected to both sources of hyperthermia under comparable conditions showed no significant difference in survival. The Do (slope of the exponential region) of the survival curve was 8.3 minutes for the water-bath treatment compared with 8.1 minutes for the microwave treatment. Similarly, the Dq (a measure of the survival curve that indexes cell repair) was 23.4 minutes for the water-bath treatment and 23.7 minutes for the microwave treatment. Intrasample uniformity of heating and accurate thermometry were found to be highly critical to the demonstration of parity.