Three distinct epitopes within the loop region of hen egg lysozyme defined with monoclonal antibodies.
Three distinct epitopes within the loop region of hen egg lysozyme defined with monoclonal antibodies.
复制标题
用单克隆抗体定义鸡蛋溶菌酶环区内的三个不同表位。
作者：
M. Darsley;A. Rees
用溶菌酶蛋白水解片段偶联牛血清白蛋白的合成偶联物免疫鸡卵溶菌酶，制备了5种特异性抗鸡卵溶菌酶环区的单克隆抗体。在定量放射免疫测定程序中，使用一组变体溶菌酶和溶菌酶的肽片段研究其精细特异性。高分辨率的鸡溶菌酶结构的知识和计算机图形的使用使得能够以一定的精度定位由抗体识别的表位。这些抗体被证明在以前被认为是单个抗原位点的地方定义了三个不同的重叠表位。这些结果与蛋白质的抗原性的当前想法和其他最近的研究有关，其中抗蛋白抗体已通过小肽免疫引起。
Five monoclonal antibodies specific for the loop region of hen egg lysozyme were prepared by immunisation with a synthetic conjugate of a proteolytic fragment of lysozyme coupled to bovine serum albumin. Their fine specificities were investigated using a panel of variant lysozymes and peptide fragments of lysozyme in a quantitative radio‐immunoassay procedure. Knowledge of the structure of hen lysozyme to high resolution and the use of computer graphics enables the localisation of the epitopes recognised by the antibodies with some precision. The antibodies were shown to define three distinct, overlapping epitopes within what was previously considered to be a single antigenic site. These results are discussed in relation to current ideas of the antigenic nature of proteins and other recent studies in which anti‐protein antibodies have been elicited by immunisation with small peptides.