A Zinc Finger Transcriptional Repressor Confers Pleiotropic Effects on Rice Growth and Drought Tolerance by Down-Regulating Stress-Responsive Genes
A Zinc Finger Transcriptional Repressor Confers Pleiotropic Effects on Rice Growth and Drought Tolerance by Down-Regulating Stress-Responsive Genes
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锌指转录抑制因子通过下调应激反应基因对水稻生长和耐旱性产生多效性
DOI：
10.1093/pcp/pcy133
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发表时间：
2018
影响因子：
4.9
通讯作者：
Huang Ji
中科院分区：
文献类型：
--
作者：
Yuan Xi;Huang Peng;Wang Ruqin;Li Haoyuan;Lv Xueqing;Duan Min;Tang Haijuan;Zhang Hongsheng;Huang Ji
C2 H2型锌指蛋白（ZFP）参与植物的发育和逆境应答。许多研究表明ZFP基因在植物抗逆中发挥着积极的作用。然而，ZFP基因的过表达通常会导致生长迟缓的副作用。在这里，我们报告了ZFP家族的一个新成员，水稻干旱响应锌指蛋白1（OsDRZ 1），积极调节水稻（Oryza sativa L.）的胁迫耐受性和植物结构。OsDRZ 1在所有组织中均有表达，并能被多种非生物胁迫诱导表达。OsDRZ 1蛋白主要定位于细胞核。与大多数报道的水稻ZFPs作为转录激活因子不同，OsDRZ 1是转录抑制因子。过量表达OsDRZ 1基因可提高水稻幼苗的耐旱性，增加游离脯氨酸的积累，减少活性氧的产生，提高抗氧化酶的活性。相比之下，OsDRZ 1的RNA干扰（RNAi）导致抗氧化反应活性降低，对干旱更加敏感。RNA测序分析表明，OsDRZ 1下调的基因大多数在干旱胁迫下表达下调，表明OsDRZ 1在调节干旱响应基因表达中起重要作用。通过实时荧光定量PCR和水稻原生质体瞬时表达分析，确定cupin基因OsGLP 1（germin-like protein 1）为OsDRZ 1的潜在靶基因之一。此外，OsDRZ 1基因的过表达不仅没有抑制水稻的生长，反而促进了水稻的生长，表明OsDRZ 1基因在抗旱作物工程中具有潜在的应用前景。
The C2H2-type zinc finger proteins (ZFPs) are involved in a wide range of plant development and stress responses. Many studies have shown the positive roles of ZFP genes in stress tolerance. However, overexpression of ZFP genes usually leads to the side effect of growth retardation. Here we report a new member of the ZFP family, Oryza sativa drought-responsive zinc finger protein 1 (OsDRZ1), positively regulating both stress tolerance and plant architecture in rice (Oryza sativa L.). OsDRZ1 was expressed throughout all tissues examined and could be induced by multiple abiotic stresses. OsDRZ1 protein was localized mostly in the nucleus. Unlike most reported rice ZFPs functioning as transcriptional activators, OsDRZ1 is a transcriptional repressor. Overexpression of OsDRZ1 in rice increased seedling drought tolerance and the transgenic plants appeared to accumulate more free proline and fewer reactive oxygen species (ROS), and elevate the activities of antioxidant enzymes. In contrast, RNA interference (RNAi) of OsDRZ1 led to lower activities of antioxidative response and more sensitivity to drought. RNA sequencing analysis revealed that the genes down-regulated by OsDRZ1 were mostly down-regulated by drought, implying the critical role of OsDRZ1 in modulating drought-responsive gene expression. A cupin gene OsGLP1 (germin-like protein1) was identified as one of the potential target genes of OsDRZ1, as suggested by real-time PCR and transient expression analysis in rice protoplasts. Moreover, overexpression of OsDRZ1 did not lead to growth inhibition but the promotion of rice growth, implying the potential application prospective of OsDRZ1 in engineering drought-tolerant crops.