Isolation and characterization of bone marrow-derived equine mesenchymal stem cells
Isolation and characterization of bone marrow-derived equine mesenchymal stem cells
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DOI：
10.2460/ajvr.68.10.1095
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发表时间：
2007-10-01
影响因子：
1
通讯作者：
Litzke, Lutz F.
中科院分区：
文献类型：
--
作者：
Arnhold, Stefan J.;Goletz, Iris;Litzke, Lutz F.
目的-分离和表征骨髓来源的马间充质干细胞 (MSC)，以用于未来可能在马中的治疗应用。样本群体-马 MSC 是从 30 匹供体马的胸骨获得的骨髓抽吸物中分离出来的。程序-细胞在胎牛血清含量为 20% 的培养基（α-最低必需培养基）中培养。分析马 MSC 特征以确定自我更新和分化能力。为了潜在的治疗应用，马 MSC 的迁移潜力被确定。采用腺病毒载体测定马MSCs的转导率。结果-马MSCs可进行培养扩增。马 MSC 在液氮中冷冻保存，不会改变形态特征。此外，马间充质干细胞在解冻后仍保持增殖和分化的能力。通过免疫细胞化学方法，可以在马 MSC 上检测到干细胞标记物 CD90 的表达。马间充质干细胞的多谱系分化潜力通过其进行脂肪形成、成骨和软骨形成分化的能力来揭示。结论和临床相关性-我们的数据表明马骨髓来源的基质细胞可以被表征。作为间充质干细胞。马间充质干细胞具有高转导率和迁移潜力，并适应培养中的支架材料。作为一种自体细胞群，马间充质干细胞可被视为用于马肌肉骨骼系统病变组织工程的有前途的细胞群。
Objective-To isolate and characterize bone marrow-derived equine mesenchymal stem cells (MSCs) for possible future therapeutic applications in horses.Sample Population-Equine MSCs were isolated from bone marrow aspirates obtained from the sternum of 30 donor horses.Procedures-Cells were cultured in medium (alpha-minimum essential medium) with a fetal calf serum content of 20%. Equine MSC features were analyzed to determine self-renewing and differentiation capacity. For potential therapeutic applications, the migratory potential of equine MSCs was determined. An adenoviral vector was used to determine the transduction rate of equine MSCs.Results-Equine MSCs can be culture-expanded. Equine MSCs undergo cryopreservation in liquid nitrogen without altering morphologic characteristics. Furthermore, equine MSCs maintain their ability to proliferate and differentiate after thawing. Immunocytochemically, the expression of the stem cell marker CD90 can be detected on equine MSCs. The multilineage differentiation potential of equine MSCs was revealed by their ability to undergo adipogenic, osteogenic, and chondrogenic differentiation.Conclusions and Clinical Relevance-Our data indicate that bone marrow-derived stromal cells of horses can be characterized. as MSCs. Equine MSCs have a high transduction rate and migratory potential and adapt to scaffold material in culture. As an autologous cell population, equine MSCs can be regarded as a promising cell population for tissue engineering in lesions of the musculoskeletal system in horses.