Quantitative high-performance liquid chromatography photo-diode array (HPLC-PDA) analysis of benzophenones and biflavonoids in eight Garcinia species

Quantitative high-performance liquid chromatography photo-diode array (HPLC-PDA) analysis of benzophenones and biflavonoids in eight Garcinia species
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DOI:
10.1016/j.jfca.2011.10.006
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发表时间:
2012-03-01
影响因子:
4.3
通讯作者:
Kennelly, Edward J.
Kennelly, Edward J.
中科院分区:
农林科学2区
文献类型:
--
作者:
Acuna, Ulyana Munoz;Dastmalchi, Keyvan;Kennelly, Edward J.

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山竹子属8种植物,包括7种可食果实,山竹子Garcinia aristata、山竹子G. hombroniana,黄毛棉G. intermedia,G. livingstonei,“超级水果”G. mangostana、山竹G. spicata和G. xanthochochlorophyll和G.使用经验证的高效液相色谱光电二极管阵列(HPLC-PDA)方法分析了可乐。三个二苯甲酮,guttiferone A,guttiferone E,和xanthochymol,和四个双黄酮amentoflavone,fukugeside,fukugetin,和volkensiflavone进行了鉴定和定量的HPLC-PDA与真实的标准,以验证其身份。中间山竹(Garcinia intermedia)的IC_(50)为60.1 ± 27.3 μ g/mL; mangostana(IC 50 = 64.3 +/- 11.3 μ g/mL)果实表现出最高的抗氧化活性。黄色素酚含量最高的是C. spicata(70.50 ± 1.10 mg/g干重),而在G. intermedia(43.00 +/- 0.30 mg/g干重),因此确定富含总酚含量。(C)2012爱思唯尔公司出版
Eight Garcinia species including seven edible fruits, Garcinia aristata, G. hombroniana, G. intermedia, G. livingstonei, the "superfruit" G. mangostana, G. spicata, and G. xanthochymus, and the wood of G. kola were analyzed using a validated high-performance liquid chromatography photo-diode array (HPLC-PDA) method. Three benzophenones, guttiferone A, guttiferone E, and xanthochymol, and four biflavonoids amentoflavone, fukugeside, fukugetin, and volkensiflavone were identified and quantified by HPLC-PDA with authentic standards to verify their identity. Garcinia intermedia (IC50 = 60.1 +/- 27.3 mu g/mL) and G. mangostana (IC50 = 64.3 +/- 11.3 mu g/mL) fruits demonstrated the highest antioxidant activity. The highest level of xanthochymol was found in C. spicata (70.50 +/- 1.10 mg/g dry weight), while the highest quantity of guttiferone A was detected in G. intermedia (43.00 +/- 0.30 mg/g dry weight), and thus was determined to be rich in total phenolic content. (C) 2012 Published by Elsevier Inc.