Coumarins and Lignans from Zanthoxylum schinifolium and Their Anticancer Activities
Coumarins and Lignans from Zanthoxylum schinifolium and Their Anticancer Activities
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DOI：
10.1021/jf403479c
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发表时间：
2013-11-13
影响因子：
6.1
通讯作者：
Kim, Young Ho
中科院分区：
文献类型：
--
作者：
Li, Wei;Sun, Ya Nan;Kim, Young Ho
花椒是一种芳香灌木，其果皮和叶片在东亚国家广泛用于烹饪应用。长期以来，它也被用于传统的东方医学，用于治疗普通感冒，胃痛，腹泻和黄疸。在这项研究中，我们鉴定了两个新的化合物，花椒苷（1）和schinifolisatin A（13），沿着23个已知的香豆素（2-12）和木脂素（14-25），从甲醇提取物的Z。[医]石杉属化合物的化学结构通过质量、1D-和2D NMR光谱确定。在三种人癌细胞系中检查了分离的化合物的抗癌效果。化合物10-12显著降低HL-60人急性早幼粒细胞白血病细胞的增殖，IC 50值为4.62-5.12 μ M。用化合物10处理PC-3前列腺癌细胞和SNU-C5结肠直肠癌细胞导致有效的抗增殖活性，IC 50值分别为4.39和6.26 μ M。此外，化合物10-12诱导三种癌细胞的凋亡。此外，细胞凋亡的诱导伴随着p-ERK 1/2 MAPK、p-AKT和c-myc水平的下调，呈时间依赖性。这些数据表明，化合物10-12是从Z. Schinifolium在癌症治疗中具有潜力。
Zanthoxylum schinifolium is an aromatic shrub, and its pericarp and leaves are widely used in culinary applications in East Asian countries. It has also long been used in traditional Oriental medicine for treating the common cold, stomach ache, diarrhea, and jaundice. In this study, we identified two new compounds, zanthoxyloside (1) and schinifolisatin A (13), along with 23 known coumarins (2-12) and lignans (14-25), from a methanol extract of the stems of Z. schinifolium. The chemical structures of the compounds were determined by mass, 1D-, and 2D NMR spectroscopy. The anticancer effects of the isolated compounds were examined in three human cancer cell lines. Compounds 10-12 significantly reduced the proliferation of HL-60 human acute promyelocytic leukemia cells with IC50 values of 4.62-5.12 mu M. Treatment of PC-3 prostate cancer cells and SNU-C5 colorectal cancer cells with compound 10 resulted in potent antiproliferative activity, with IC50 values of 4.39 and 6.26 mu M, respectively. Also, compounds 10-12 induced the apoptosis of three cancer cells. Furthermore, the induction of apoptosis was accompanied by down-regulation of p-ERK1/2 MAPK, p-AKT, and c-myc levels, in a time-dependent manner. These data suggested that compounds 10-12 from Z. schinifolium have potential in cancer treatment.