Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii
Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii
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DOI：
10.1098/rsob.180139
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发表时间：
2018-10-01
期刊：
影响因子：
5.8
通讯作者：
Arendt, Detlev
中科院分区：
文献类型：
--
作者：
Chartier, Thomas F.;Deschamps, Joran;Arendt, Detlev
化学检测是海洋和陆地动物各种行为的关键。海洋物种，虽然高度多样性，一直代表性不足，到目前为止，在化学传感系统的研究，我们的知识主要涉及检测空气中的线索。因此，最好采取更广泛的比较办法。海洋环节动物蠕虫具有丰富的行为技能，是化学感觉的有吸引力的模型。在这里，我们研究海洋蠕虫Platynereis dumerilii提供的第一个全面调查头部化学感觉器官生理环节动物。通过结合微流体和钙成像，我们记录了化学刺激后早期青少年整个头部的神经元活动。我们发现，Platynereis使用四种类型的器官来检测刺激，如醇，酯，氨基酸和糖。与反应差异更大的颈部器官或触须相比，触角是主要的化学感受器官。我们报告化学诱发的活动，可能的下游脑区，包括蘑菇体（MB），这是解剖学和分子相似的昆虫MB。我们的结论是化学感觉是海洋环节动物的一个主要的感觉方式，并提出早期的Platynereis青少年作为模型来研究环节动物的化学感觉系统。
Chemical detection is key to various behaviours in both marine and terrestrial animals. Marine species, though highly diverse, have been under-represented so far in studies on chemosensory systems, and our knowledge mostly concerns the detection of airborne cues. A broader comparative approach is therefore desirable. Marine annelid worms with their rich behavioural repertoire represent attractive models for chemosensation. Here, we study the marine worm Platynereis dumerilii to provide the first comprehensive investigation of head chemosensory organ physiology in an annelid. By combining microfluidics and calcium imaging, we record neuronal activity in the entire head of early juveniles upon chemical stimulation. We find that Platynereis uses four types of organs to detect stimuli such as alcohols, esters, amino acids and sugars. Antennae are the main chemosensory organs, compared to the more differentially responding nuchal organs or palps. We report chemically evoked activity in possible downstream brain regions including the mushroom bodies (MBs), which are anatomically and molecularly similar to insect MBs. We conclude that chemosensation is a major sensory modality for marine annelids and propose early Platynereis juveniles as a model to study annelid chemosensory systems.