Synergistic removal performance and mechanism in denitrification system under phenol stress
Synergistic removal performance and mechanism in denitrification system under phenol stress
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DOI：
10.1016/j.jece.2023.110767
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发表时间：
2023-08
影响因子：
7.7
通讯作者：
Lei Feng;Yi Han;Jianzeng Niu;Jianbo Guo;Jianbing Zhang;Haibo Li;Yanan Hou;Yuanyuan Song
中科院分区：
文献类型：
--
作者：
Lei Feng;Yi Han;Jianzeng Niu;Jianbo Guo;Jianbing Zhang;Haibo Li;Yanan Hou;Yuanyuan Song
基于异养反硝化原理，研究了苯酚胁迫下反硝化系统中苯酚和NO3--N的协同去除机理。UASB反应器运行119 d后，成功实现了以苯酚为唯一碳源的反硝化工艺，苯酚和NO3--N的去除率均在99.5%以上。结果表明，苯酚胁迫提高了ATP水平，提高了反硝化和苯酚降解的关键酶活性。酚胁迫还刺激细胞膜透性的改善，导致电子传递系统活性的增加。同时，苯酚胁迫促进了N-酰基高丝氨酸内酯（AHLs）的分泌，AHLs通过调节胞外多糖（EPS）来提高细菌活性，缓解外界环境冲击。此外，AHL介导的群体感应（QS）还可能通过调控微生物结构的变化，使整个反硝化系统中的微生物群落达到最优化。因此，协同去除机制被提出来解释反硝化系统在苯酚胁迫下的优异性能。本研究为高浓度含酚废水的处理及协同去除机理提供了有益的参考。
Based on the principle of heterotrophic denitrification, the synergistic removal mechanism of phenol and NO3--N in denitrification system under phenol stress was investigated. The denitrification process with phenol as the only carbon source was successfully achieved by running the UASB reactor for 119 days, which had excellent phenol and NO3--N removal efficiency (over 99.5%). Results showed that phenol stress enhanced the ATP level, key enzyme activities to increase denitrification and phenol degradation. Phenol stress also stimulated improvement of cell membrane permeability, which led to the increase of electron transport system activity. Meanwhile, phenol stress promoted the secretion of N-acylhomoserine lactones (AHLs), which increased bacterial activity by modulating EPS to mitigate the external environmental shock. Besides, AHLs-mediated quorum sensing (QS) might regulated the changes of microbial structural, resulting in the optimization of microbial communities within the whole denitrification system. Therefore, a synergistic removal mechanism was proposed to elucidate the excellent performance of the denitrification system under phenol stress. This work provided serviceable information for treatment of high concentration phenol wastewater and the synergistic removal mechanism.