Pyrophosphate:D-fructose 6-phosphate 1-phosphotransferase. A new enzyme with the glycolytic function of 6-phosphofructokinase.
Pyrophosphate:D-fructose 6-phosphate 1-phosphotransferase. A new enzyme with the glycolytic function of 6-phosphofructokinase.
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DOI：
10.1016/s0021-9258(19)42029-2
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发表时间：
1974-12
期刊：
影响因子：
--
通讯作者：
Richard E. Reeves;Dorothy J. South;H. J. Blytt;Lionel G. Warren
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文献类型：
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作者：
Richard E. Reeves;Dorothy J. South;H. J. Blytt;Lionel G. Warren
溶组织内阿米巴的主要酶利用无机焦磷酸盐作为磷酸盐供体，从6-磷酸果糖产生1,6-二磷酸果糖。在其逆反应中，该酶利用正磷酸盐和果糖二磷酸盐生成无机焦磷酸盐和果糖 6-磷酸盐。该酶已从 amebal 匀浆中纯化 100 倍。在 pH 7 时，使用 2.5 mmMgCl，观察到以下 Km 值：焦磷酸盐，14 µm； 6-磷酸果糖，38 µm；果糖 1,6-二磷酸，18 µm；和正磷酸盐，800 µm。在该 pH 值和镁离子浓度下，反应的最大速度在每个方向上大致相同。阿米巴细胞内焦磷酸盐的平均浓度为 0.18 mm，该值比新酶的焦磷酸盐 Km 高 13 倍。这种酶使大肠杆菌糖酵解途径中已知酶的数量增加到三种。利用或产生焦磷酸盐的组织溶解病。这种新酶的俗名是 6-磷酸果糖激酶 (PPi)。
The major enzyme ofEntamoeba histolyticawhich produces fructose 1,6-diphosphate from fructose 6-phosphate utilizes inorganic pyrophosphate as phosphate donor. In its reverse reaction the enzyme utilizes orthophosphate and fructose diphosphate to yield inorganic pyrophosphate and fructose 6-phosphate. The enzyme has been purified 100-fold from an amebal homogenate. At pH 7 with 2.5 mmMgCl the followingKmvalues were observed: pyrophosphate, 14 µm; fructose 6-phosphate, 38 µm; fructose 1,6-diphosphate, 18 µm; and orthophosphate, 800 µm. At this pH and magnesium ion concentration the maximum velocity of the reaction was approximately the same in each direction. The average intracellular concentration of pyrophosphate in the ameba is 0.18 mm, a value 13-fold greater than the pyrophosphateKmfor the new enzyme. This enzyme raises to three the number of known enzymes in the glycolytic pathway ofE. histolyticawhich utilize or produce pyrophosphate. The trivial name proposed for the new enzyme is 6-phosphofructokinase (PPi).