Abortive replication of Bombyx mori nucleopolyhedrovirus in Sf9 and High Five cells:: Defective nuclear transport of the virions
Abortive replication of Bombyx mori nucleopolyhedrovirus in Sf9 and High Five cells:: Defective nuclear transport of the virions
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DOI：
10.1016/j.virol.2005.11.043
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发表时间：
2006-04-10
期刊：
影响因子：
3.7
通讯作者：
Kobayashi, M
中科院分区：
文献类型：
--
作者：
Katou, Y;Ikeda, M;Kobayashi, M
尽管家蚕核型多角体病毒（BmNPV）和苜蓿银纹夜蛾核型多角体病毒（AcMNPV）有着密切的遗传关系，但它们的寄主范围不同。在此，在Sf 9和High Five细胞中检查BmNPV复制，所述细胞对于BmNPV感染是非生产性的，但支持高滴度的AcMNPV复制。重组BmNPV vBm/gfp/lac病毒感染Sf 9和High Five细胞后，只有少数细胞表达EGFP，而vBm/gfp/lac病毒感染后，则有大量细胞表达EGFP。免疫细胞化学分析表明，BmNPV未被导入细胞核，而含有AcMNPV gp 64的重组BmNPV vBmDelta 64/ac-gp 64则被导入细胞核，在High Five细胞中产生子代病毒粒子，而在High Five细胞中未产生子代病毒粒子。这些结果表明，由于BmNPVGP 64功能不足而导致的感染病毒粒子的核输入缺陷与Sf 9和High Five细胞中BmNPV的复制受限有关。(c)2005年爱思唯尔公司All rights reserved.
Despite close genetic relationship, Bombyx mori nucleopolyhedrovinis (BmNPV) and Autographa californica multicapsid NPV (AcMNPV) display a distinct host range property. Here, BmNPV replication was examined in Sf9 and High Five cells that were nonproductive for BmNPV infection but supported high titers of AcMNPV replication. Recombinant BmNPV vBm/gfp/lac, containing bm-iel promoter-driven egfp showed that few Sf9 and High Five cells infected with vBm/gfp/lac expressed EGFP, while large proportion of EGFP-expressing cells was observed when transfected with vBm/gfp/lac DNA. Immunocytochemical analysis showed that BmNPV was not imported into the nucleus of these two cell lines, while recombinant BmNPV, vBm Delta 64/ac-gp64 possessing AcMNPV gp64 was imported into the nucleus, yielding progeny virions in High Five cells, but not so cells. These results indicate that the defective nuclear import of infected virions due to insufficient BmNPV GP64 function is involved in the restricted BmNPV replication in Sf9 and High Five cells. (c) 2005 Elsevier Inc. All rights reserved.