Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: effect on sperm velocity.
Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: effect on sperm velocity.
复制标题
使用红外激光光阱在三个维度上控制人类精子的显微操作：对精子速度的影响。
DOI：
10.1016/s0015-0282(16)54926-7
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发表时间：
1992
影响因子：
6.7
通讯作者：
Bonder,EM
中科院分区：
文献类型：
--
作者：
Colon,JM;Sarosi,P;McGovern,PG;Askin,A;Dziedzic,JM;Skurnick,J;Weiss,G;Bonder,EM
使用1.06微米Nd: YAG激光陷阱可以在三维空间中对单个人类精子进行微操作。以65到85微米/秒的速度游动的单个精子可以在40兆瓦的功率下被捕获120秒而不会对速度产生有害影响。尽管有必要进一步评估激光对精子特定功能的影响，但我们的数据表明，将操作时间减少到最小将增加微操作程序的安全性。激光诱捕器可能通过促进单个精子的选择性运输而在辅助生殖技术中发挥作用。
Individual human sperm can be micromanipulated in three dimensions using a 1.06 microns Nd: YAG laser trap. Single sperm swimming with velocities in the range of 65 to 85 microns/sec can be trapped with 40 mW of power through 120 seconds without a deleterious effect on velocity. Even though it will be necessary to further evaluate the effects of laser light on specific functions of sperm, our data suggest that decreasing the time of manipulation to a minimum will increase the safety of the micromanipulation procedures. Laser traps may play a role in assisted reproductive technology by facilitating the selective transport of individual sperm.