Effects of Euro-Collins, University of Wisconsin, and new extracellular-type trehalase-containing Kyoto solutions in an ex vivo rat lung preservation model
Effects of Euro-Collins, University of Wisconsin, and new extracellular-type trehalase-containing Kyoto solutions in an ex vivo rat lung preservation model
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DOI：
10.1097/00007890-199611150-00004
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发表时间：
1996-11-15
期刊：
影响因子：
6.2
通讯作者：
Wada, H
中科院分区：
文献类型：
--
作者：
Fukuse, T;Hirata, T;Wada, H
背景我们以前曾报道过以海藻糖为基础的细胞外型京都（ET-K）保存液在肺保存中的作用，现在，我们研制了一种新的ET-K保存液，在ET-K保存液中加入三种物质--N-乙酰半胱氨酸、二丁酰环磷酸腺苷和硝酸甘油，研究了新的ET-K保存液在肺保存中的作用。并使用离体大鼠再灌注模型将其与Euro-Collins（EC）和威斯康星州大学（UW）溶液进行比较。灌注回路由从三只肝素化大鼠获得的30 ml新鲜混合静脉血启动。通过双头滚子泵，血液从静脉血储库通过肺动脉灌注到被检查的肺中。肺流出物以相同的流速返回到脱氧器新鲜肺。实验分为4组，新鲜组（n=6）用生理盐水灌流，其余各组（2组：新ET-K组（n=6）; UW组（n = 6）; EC组（n=6）分别用新ET-K+前列腺素E1（PGE 1）、UW + PGE 1、EC + PGE 1灌流。保存17 h后，对保存肺进行再灌注。在EC组的所有6只动物中，在再灌注后20 min，由于肺水肿导致气管内导管中的渗出物，实验肺的通气被中断。新ET-K组和UW组的分流率、肺动脉压和吸气峰压均明显优于EC组，但与新鲜组基本相当。新ET-K液保存后肺功能优于EC液，与UW液相当。这种新的解决方案有望有助于增加临床肺移植的供体肺。此外，该离体大鼠肺再灌注模型操作简单，可靠性高，可广泛应用于肺保护的研究。
Background. We have previously reported the effects of trehalose-based extracellular-type Kyoto (ET-K) solution in lung preservation, Now, we have developed a new ET-K solution by adding three substances-N-acetyl cysteine, dibutyryl cyclic AMP, and nitroglycerin, to ET-K solution, We studied the effects of new ET-K solution in lung preservation, and compare it with Euro-Collins (EC) and University of Wisconsin (UW) solutions using an ex vivo rat reperfusion model.Methods. The perfusion circuit was initiated by 30 ml of fresh mixed venous blood obtained from three haparinized rats. By means of a double-head roller pump, the blood passed from the venous blood reservoir through the pulmonary artery to be perfused in the examined lung. The lung effluent was returned at the same flow rate to the deoxygenator fresh lung. Four experimental groups were allocated, In group 1 (fresh group, n=6), lung was flushed with saline and reperfused immediately, In the other groups (group 2: new ET-K group, n=6; group 3: UW group, n=6; and group 4: EC group, n=6), lung was flushed with the new ET-K and prostanglandin E1 (PGE1), UW and PGE1, and EC and PGE1, respectively. After 17-hr preservation, the preserved lung was reperfused.Results. In all six animals of the EC group, ventilation of the experimental lung was discontinued at 20 min after reperfusion because of the exudate in the endotracheal tube that resulted from pulmonary edema. The shunt fraction, pulmonary arterial pressure, and peak inspiratory pressure in the new ET-K and UW groups were significantly better than those in the EC group, but were almost equal to those in the fresh group.Conclusion. The postpreservation pulmonary functions with the new ET-K solution were better than those with, the EC solution, and were equal to those with the UW solution. This new solution is expected to contribute to the increase in donor lungs for clinical lung transplantation. In addition, this ex vivo rat reperfusion model is simple and highly reliable, and can be widely used in the studies of pulmonary preservation.