Pepitope: epitope mapping from affinity-selected peptides
Pepitope: epitope mapping from affinity-selected peptides
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DOI：
10.1093/bioinformatics/btm493
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发表时间：
2007-12-01
期刊：
影响因子：
5.8
通讯作者：
Pupko, Tal
中科院分区：
文献类型：
--
作者：
Mayrose, Itay;Penn, Osnat;Pupko, Tal
确定抗体结合的表位是许多免疫学应用的核心，如药物设计和疫苗开发。Pepitope服务器是一个基于网络的工具，旨在基于一组针对感兴趣的单克隆抗体亲和选择的肽来预测不连续的表位。服务器实现了三种不同的表位映射算法：PepSurf、Mapitope以及两者的组合。这些算法背后的基本原理是肽集在物理化学性质和空间组织方面模仿真正的表位。当抗原的三维（3D）结构已知时，这些肽中的信息可用于计算推断相应的表位。开发了一个用户友好的网络界面和图形工具，可以查看预测的表位。Pepitope也可以用于推断免疫学背景之外的其他类型的蛋白质相互作用，并作为将线性序列对齐到3D结构的通用工具。
Identifying the epitope to which an antibody binds is central for many immunological applications such as drug design and vaccine development. The Pepitope server is a web-based tool that aims at predicting discontinuous epitopes based on a set of peptides that were affinity-selected against a monoclonal antibody of interest. The server implements three different algorithms for epitope mapping: PepSurf, Mapitope, and a combination of the two. The rationale behind these algorithms is that the set of peptides mimics the genuine epitope in terms of physicochemical properties and spatial organization. When the three-dimensional (3D) structure of the antigen is known, the information in these peptides can be used to computationally infer the corresponding epitope. A user-friendly web interface and a graphical tool that allows viewing the predicted epitopes were developed. Pepitope can also be applied for inferring other types of proteinprotein interactions beyond the immunological context, and as a general tool for aligning linear sequences to a 3D structure.