BETA-2-ADRENOCEPTOR DESENSITIZATION IN CULTURED HUMAN AIRWAY SMOOTH-MUSCLE
BETA-2-ADRENOCEPTOR DESENSITIZATION IN CULTURED HUMAN AIRWAY SMOOTH-MUSCLE
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DOI：
10.1042/cs0840151
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发表时间：
1993-02-01
期刊：
影响因子：
6
通讯作者：
WIDDOP, S
中科院分区：
文献类型：
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作者：
HALL, IP;DAYKIN, K;WIDDOP, S
1.在原代培养的人气道平滑肌细胞上，研究了环磷酸腺苷对异丙肾上腺素反应的调控机制。在这些细胞中，异丙肾上腺素通过β2肾上腺素受体刺激诱导浓度相关的环状AMP的形成。预先用不同浓度的异丙肾上腺素(1-16h)、Forsklin、前列腺素E_2或一种稳定的环磷酸腺苷类似物孵育细胞，均可使cAMP对随后用异丙肾上腺素攻击的反应产生浓度相关的脱敏作用(1摩尔/L异丙肾上腺素作用16h后最大降幅为85%)。前列腺素E_2诱导的脱敏作用超过2小时(44%)，环磷酸腺苷水平的升高与摩尔/L异丙肾上腺素相似(P<0.05)。
1. The mechanisms underlying control of cyclic AMP responses to isoprenaline were studied in primary cultures of human airway smooth muscle cells. In these cells, isoprenaline induced concentration-related cyclic AMP formation via beta2-adrenoceptor stimulation.2. Prior incubation of cells with varying concentrations of isoprenaline (1-16 h), forskolin, prostaglandin E2 or a stable analogue of cyclic AMP all produced concentration-related desensitization of cyclic AMP responses to subsequent challenge with isoprenaline (maximum reduction with 1 mumol/l isoprenaline, 85% after 16 h). The desensitization induced over 2h (44%) by a concentration of prostaglandin E2 which gave a similar rise in cyclic AMP levels to 1 mumol/l isoprenaline was significantly less (P