Genetic strain variations in the metaphase-II phenotype of mouse oocytes matured in vivo or in vitro
Genetic strain variations in the metaphase-II phenotype of mouse oocytes matured in vivo or in vitro
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DOI：
10.1530/rep.1.00558
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发表时间：
2005-12-01
期刊：
影响因子：
3.8
通讯作者：
Albertini, DF
中科院分区：
文献类型：
--
作者：
Ibáñez, E;Sanfins, A;Albertini, DF
遗传和表观遗传因素之间的相互作用在哺乳动物胚胎生产策略中起着核心作用，该策略依赖于菌株背景特征的离体或体内操作。在这项研究中，我们研究了遗传背景和表型特性的小鼠中期-II（M-II）卵母细胞在体内（IVO）或体外条件下成熟，无论是在基础（IVM）或补充（IVM +）培养基之间的关系。在诱导超数排卵的近交系（C57 BL/6）、远交系（CF-1，Black Swiss，NU/NU）和杂交系（B6 D2 F1）中，除了Black Swiss卵母细胞纺锤体较大且不对称外，细胞质微管组织中心（MTOC）的数量与纺锤体的大小和形状无关。当卵母细胞在培养中成熟时，M-II卵母细胞的减数分裂纺锤体和细胞质表型特性相对于体内条件和菌株之间受到影响。具体而言，措施减数分裂纺锤体的大小，形状，极性pericentrin分布和细胞质MTOC数都揭示了特征的变化。有趣的是，在IVM时观察到的细胞质MTOC数量的总体减少伴随着纺锤体和极体尺寸的总体增加。IVM +条件下的成熟导致细胞质MTOC数量进一步减少，但纺锤体和极体特征介于IVO和IVM之间。这些母源卵母细胞的表型特性如何与生殖力的预测评估联系起来仍有待确定。
The interplay between genetic and epigenetic factors plays a central role in mammalian embryo production strategies that superimpose ex vivo or in vivo manipulations upon strain background characteristics. In this study, we examined the relationship between genetic background and the phenotypic properties of mouse metaphase-II (M-II) oocytes that were matured under in vivo (IVO) or in vitro conditions, either in a basal (IVM) or a supplemented (IVM +) medium. Differences existed amongst inbred (C57BL/6), outbred (CF-1, Black Swiss, NU/NU) and hybrid lines (B6D2F1) induced to superovulate with regard to cytoplasmic microtubule organizing center (MTOC) number but not spindle size or shape, except for larger and asymmetrical spindles in Black Swiss oocytes. When oocytes were matured in culture, meiotic spindle and cytoplasmic phenotypic properties of M-II oocytes were affected relative to in vivo conditions and between strains. Specifically, measures of meiotic spindle size, shape, polar pericentrin distribution and cytoplasmic MTOC number all revealed characteristic variations. Interestingly, the overall reduction in cytoplasmic MTOC number noted upon IVM was concomitant with an overall increase in spindle and polar body size. Maturation under IVM + conditions resulted in a further decrease in cytoplasmic MTOC number, but spindle and polar body characteristics were intermediate between IVO and IVM. How these oocyte phenotypic properties of maternal origin may be linked to predictive assessments of fecundity remains to be established.