Development and life cycle of the parthenogenetically activated sea urchin embryo.
Development and life cycle of the parthenogenetically activated sea urchin embryo.
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孤雌激活海胆胚胎的发育和生命周期。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
R. Steinhardt
中科院分区：
文献类型：
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作者：
B. Brandriff;R. Hinegardner;R. Steinhardt
报道了一种诱导海胆卵孤雌发育的方法，这种海胆以前不能人工激活。NH 4 OH或钙离子载体A23187用作活化剂，然后进行高渗处理。离子载体在激活大量未受精卵方面是上级的，而NH 4 OH产生能够经历原肠胚形成的胚胎的百分比更大。这些人工激活的卵产生的摄食幼虫和海胆都是二倍体。所有已确定性别的个体均为女性。这些完全纯合的生物体的生存能力与其受精的对应物相比是低的。
A method is reported for inducing parthenogenetic development in eggs of the sea urchin Lytechinus pictus, a species which previously could not be artificially activated. NH4OH or the calcium ionophore A23187 are used as activating agents followed by hypertonic treatment. The ionophore is superior in activating large numbers of unfertilized eggs, whereas NH4OH produces a larger percent of embryos able to undergo gastrulation. Both feeding larvae and urchins arising from these artificially activated eggs are diploid. All individuals in which sex has been identified have been female. The viability of these completely homozygous organisms is low compared to their fertilized counterparts.