Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast
Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast
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DOI：
10.1101/gad.11.24.3432
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发表时间：
1997-12-15
影响因子：
10.5
通讯作者：
Cyert, MS
中科院分区：
文献类型：
--
作者：
Stathopoulos, AM;Cyert, MS
钙调神经磷酸酶是一种保守的钙/钙调素依赖性蛋白磷酸酶，在钙信号转导中起着关键作用。我们描述了酵母中钙调神经磷酸酶介导的反应的新成分，即编码β-1，3葡聚糖合成酶的FKS2的钙诱导转录激活。FKS2启动子的24bp区域被定义为足以对最小的启动子进行钙调神经磷酸酶依赖的转录诱导，并被命名为CDRE(钙调神经磷酸酶依赖的反应元件)。CRZ1(YNL027w)的产物被鉴定为CDRE驱动的转录激活剂。Crz1p含有锌指基序，并与CDRE特异结合。遗传分析显示，crz1 Delta突变体细胞表现出与钙调神经磷酸酶突变体相似的几种表型，而CRZ1在钙调神经磷酸酶突变体中的过表达抑制了这些表型。这些结果表明，Crz1p在钙调神经磷酸酶下游发挥作用，以影响多种钙调神经磷酸酶依赖的反应。此外，钙调神经磷酸酶依赖的FKS2转录诱导对钙、α-因子和Na+的反应被发现需要CRZ1。此外，我们还发现，依赖于钙调神经磷酸酶的PMR2和PMC1的转录调控需要CRZ1。然而，PMR2和PMC1的转录只被激活FKS2转录的处理的一部分激活。因此，在多种信号的响应下，钙调神经磷酸酶通过Crz1p转录因子在酵母中差异调节几个靶基因的表达。
Calcineurin is a conserved Ca2+/calmodulin-dependent protein phosphatase that plays a critical role in Ca2+ signaling. We describe new components of a calcineurin-mediated response in yeast, the Ca2+-induced transcriptional activation of FKS2, which encodes a beta-1,3 glucan synthase. A 24-bp region of the FKS2 promoter was defined as sufficient to confer calcineurin-dependent transcriptional induction on a minimal promoter in response to Ca2+ and was named CDRE (for calcineurin-dependent response element). The product of CRZ1 (YNL027w) was identified as an activator of CDRE-driven transcription. Crz1p contains zinc finger motifs and binds specifically to the CDRE. Genetic analysis revealed that crz1 Delta mutant cells exhibit several phenotypes similar to those of calcineurin mutants and that overexpression of CRZ1 in calcineurin mutants suppressed these phenotypes. These results suggest that Crz1p functions downstream of calcineurin to effect multiple calcineurin-dependent responses. Moreover, the calcineurin-dependent transcriptional induction of FKS2 in response to Ca2+, alpha-factor, and Na+ was found to require CRZ1. In addition, we found that the calcineurin-dependent transcriptional regulation of PMR2 and PMC1 required CRZ1. However, transcription of PMR2 and PMC1 was activated by only a subset of the treatments that activated FKS2 transcription. Thus, in response to multiple signals, calcineurin acts through the Crz1p transcription factor to differentially regulate the expression of several target genes in yeast.