Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: General implications and practical use in endodontic antimicrobial therapy
Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: General implications and practical use in endodontic antimicrobial therapy
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DOI：
10.1128/jcm.43.10.5332-5337.2005
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发表时间：
2005-10-01
影响因子：
9.4
通讯作者：
Conrads, G
中科院分区：
文献类型：
--
作者：
Horz, HP;Vianna, ME;Conrads, G
通过使用ARB系统发育软件包以及包含41,000个16S rRNA基因序列的数据库，对10种先前发表的“通用”聚合酶链反应（PCR）检测方法进行重新检验，我们发现它们在细菌域的覆盖范围上存在很大差异。我们评估了覆盖范围最广的实时定量PCR方案在衡量牙髓治疗抗菌效果方面的功效。
By reexamining 10 previously published "universal" PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.