The Effects of Ultraviolet Eye Irradiation on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice
The Effects of Ultraviolet Eye Irradiation on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice
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DOI：
10.1111/php.12620
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发表时间：
2016-09-01
影响因子：
3.3
通讯作者：
Sato, Eisuke F.
中科院分区：
文献类型：
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作者：
Hiramoto, Keiichi;Yamate, Yurika;Sato, Eisuke F.
紫外线（UV）眼睛照射使肠道细胞变性。本研究探讨了UVA和UVB对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎的作用。我们制作了溃疡性结肠炎小鼠模型，给予DSS 5天，并在DSS治疗期间每天用UVB或UVA照射眼睛。UVB眼部照射可使dss诱导的溃疡性结肠炎恶化。相反，UVA眼部照射可改善症状。促肾上腺皮质激素（ACTH）、促肾上腺皮质激素释放激素（CRH）、尿皮质素2、白细胞介素(IL)-18、IL-6和组胺水平在UVB眼睛照射后升高。相比之下，UVA/ dss处理小鼠血液中的-内啡肽水平升高，尿皮质素2、肿瘤坏死因子(TNF)-和组胺水平降低。此外，在结肠中，UVB/ dss处理小鼠的黑素皮质素-2受体（melanocortin-2 receptor, MC2R）表达增加，而UVA/ dss处理小鼠的-阿片受体表达增加。当给予ACTH抑制剂时，UVB眼睛照射导致dss治疗的溃疡性结肠炎恶化，而使用-阿片受体拮抗剂后，UV眼睛照射的效果消失。这些结果表明，紫外线眼照射在dss诱导的溃疡性结肠炎中起重要作用。
Ultraviolet (UV) eye irradiation denatures the cells of the intestine. This study examined the action of UVA and UVB on dextran sodium sulfate (DSS)-induced ulcerative colitis. We produced a mouse model of ulcerative colitis by administering DSS for 5 days and irradiated the eye with UVB or UVA for each day of the DSS treatment period. DSS-induced ulcerative colitis was deteriorated by the UVB eye irradiation. Conversely, the symptoms improved with UVA eye irradiation. The levels of adrenocorticotropic hormone (ACTH), corticotropin-releasing hormone (CRH), urocortin 2, interleukin (IL)-18, IL-6 and histamine in the blood increased after the UVB eye irradiation of DSS-treated mice (UVB/DSS-treated mice). In contrast, the -endorphin level in the blood of the UVA/DSS-treated mice increased and the levels of urocortin 2, tumor necrosis factor (TNF)- and histamine decreased. Furthermore, in the colon, the expression of melanocortin-2 receptors (MC2R) increased in the UVB/DSS-treated mice, while the expression of -opioid receptors increased in the UVA/DSS-treated mice. When an ACTH inhibitor was administered, UVB eye irradiation caused the deterioration of DSS-treated ulcerative colitis, while the effect of UV eye irradiation disappeared with a -opioid receptor antagonist. These results suggested that UV eye irradiation plays an important role in DSS-induced ulcerative colitis.