The function of silberblick in the positioning of the eye anlage in the zebrafish embryo
The function of silberblick in the positioning of the eye anlage in the zebrafish embryo
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DOI：
10.1006/dbio.1997.8511
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发表时间：
1997-04-01
影响因子：
2.7
通讯作者：
NussleinVolhard, C
中科院分区：
文献类型：
--
作者：
Heisenberg, CP;NussleinVolhard, C
斑马鱼和其他脊椎动物一样，最初固定在神经板内的一只眼睛在形态形成过程中必须分裂，才能发育成两只分开的眼睛。已有研究表明，神经板内中线组织的前移参与了双眼的双侧化。最近发现的Silberblick(Slb)基因突变导致眼睛不完全分离。在原肠发育和早期体细胞发生过程中，SLB胚胎的中枢神经系统(CNS)腹侧中线和下层的轴向中胚层变短变宽。而野生型胚胎在原肠发育结束时，中枢神经系统腹侧中线延伸到神经板的前缘，而SLB的中枢神经腹侧中线的前端与神经板的前缘之间存在间隙。为了探讨SLB腹侧CNS中线缩短的原因，我们测定了标记的CNS中线细胞在不同发育阶段的野生型和SLB胚胎中的命运。在SLB中，腹侧CNS中线细胞的前向迁移受损，这表明这些细胞的迁移是腹侧CNS中线延长所必需的。在SLB中，中枢神经系统腹侧中线的前部缩短导致视柄的内侧而不是双侧的诱导，随后在发育后期眼睛部分融合。对SIB表型的分析表明，神经板内中线细胞的前向迁移是最初单一眼野的适当诱导和随后的双侧化所必需的。因此，这些发现可能为阐明神经板形态发生在眼睛定位中的作用提供了一个起点。(C)1997年学术出版社。
In zebrafish, as in other vertebrates, an initially singular eye held within the neural plate has to split during morphogenesis to allow the development of two separated eyes. It has been suggested that anterior progression of midline tissue within the neural plate is involved in the bilateralization of the eye held. Mutations in the recently identified silberblick (slb) gene cause an incomplete separation of the eyes. During gastrulation and early somitogenesis, the ventral midline of the central nervous system (CNS) together with the underlying axial mesendoderm is shortened and broadened in slb embryos. While in wild-type embryos the ventral CNS midline extends to the anterior limit of the neural plate at the end of gastrulation, there is a gap between the anterior tip of the ventral CNS midline and the anterior edge of the neural plate in slb. To investigate the cause for the shortening of the ventral CNS midline in slb we determined the fate of labeled ventral CNS midline cells in wild-type and slb embryos at different stages of development. In slb, anterior migration of ventral CNS midline cells is impaired, which indicates that migration of these cells is needed for elongation of the ventral CNS midline. The anterior shortening of the ventral CNS midline in slb leads to medial instead of bilateral induction of optic stalks followed by a partial fusion of the eyes at later developmental stages. The analysis of the sIb phenotype indicates that anterior migration of midline cells within the neural plate is required for proper induction and subsequent bilateralization of an initially singular eye field. These findings may therefore provide a starting point in elucidating the role of neural plate morphogenesis in positioning of the eyes. (C) 1997 Academic Press.