Screening of lactic acid bacteria producing folate and their potential use as adjunct cultures for cheese bio-enrichment
Screening of lactic acid bacteria producing folate and their potential use as adjunct cultures for cheese bio-enrichment
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DOI：
10.1093/femsle/fnaa059
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发表时间：
2020-05-01
影响因子：
2.1
通讯作者：
Brasca, Milena
中科院分区：
文献类型：
--
作者：
Albano, Clara;Silvetti, Tiziana;Brasca, Milena
乳酸菌可以通过原位强化的方法提高食品中叶酸的含量。筛选70个LAB在de Man、Rogosa和Sharpe/M17肉汤中生长期间产生叶酸的能力。干酪乳杆菌、植物乳杆菌、副干酪乳杆菌亚种。paracasei、鼠李糖乳杆菌、德氏乳杆菌subsp.保加利亚链球菌、嗜热链球菌、乳酸乳球菌乳酸亚种（Lactococcus lactis subsp.乳酸肠球菌、屎肠球菌和乳酸肠球菌能够在培养基中合成叶酸，即使程度不同。通过微生物测定法进一步分析了47株产叶酸菌株的总叶酸、细胞外叶酸和细胞内叶酸。屎肠球菌VC 223和E.乳酸菌BT 161在培养基中分别能产生123，625.74 +/- 8.00 ng/ml和384.22 +/- 5.00 ng/ml的叶酸。进一步检测了五种菌株在奶酪中合成叶酸的能力。叶酸含量随成熟度的增加而增加，当L.干酪素VC 199处理60 d后，其转化率分别为108%和113%; paracasei SE 160和E.乳酸菌BT 161含量均超过100 ng/100 g。结果鼓励使用特定的LAB来获得天然叶酸生物富集乳制品，从而改善叶酸的摄入量。
Lactic acid bacteria (LAB) can be used to increase the folate in foods by in situ fortification. Seventy LAB were screened for their ability to produce folate during growth in de Man, Rogosa and Sharpe/M17 broth. Lactobacillus casei, Lactobacillus plantarum, Lactobacillus paracasei subsp. paracasei, Lactobacillus rhamnosus, Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, Lactococcus lactis subsp. lactis, Enterococcus faecium and Enterococcus lactis were able to synthetize folates in the medium, even if to a different extent. The 47 folate-producing strains were further analyzed by microbiological assay, for total, extra and intracellular folate. Enterococcus faecium VC223 and E. lactis BT161 were able to produce in cultural medium 123,625.74 +/- 8.00 ng/ml and 384.22 +/- 5.00 ng/ml of folate, respectively. Five strains were further examined for their ability to synthesize folate in cheese. The folate content increased with ripening up to by 54% after 30 d when L. casei VC199 was used and up to 108% and 113% after 60 d, with L. paracasei SE160 and E. lactis BT161 respectively exceeding 100 ng/100g. Results encourage the use of specific LAB to obtain natural folate bio-enriched dairy products improving folate intake.