A CMOS chip for individual cell manipulation and detection
A CMOS chip for individual cell manipulation and detection
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DOI：
10.1109/jssc.2003.819171
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发表时间：
2003-12-01
影响因子：
5.4
通讯作者：
Guerrieri, R
中科院分区：
文献类型：
--
作者：
Manaresi, N;Romani, A;Guerrieri, R
在个体基础上操纵活细胞群体对于研究细胞之间复杂的相互作用是必不可少的。我们提出了一种将介电泳致动器和光学传感器集成在硅上的新方法，该方法允许；我们必须完成这项任务。本文提出的器件是一个8 x 8 mm(2)芯片，采用双聚三金属0.35 μ m CMOS技术实现，具有102,400个驱动电极，排列在320 x 320, 20 μ m x 20 μ m微点阵列中，每个微点包括寻址逻辑，用于电极编程的嵌入式存储器和光学传感器。该芯片可以通过软件控制超过1万个活细胞的位移，使生物学家能够设计复杂的相互作用协议。否则是不可能做到的。这种操作不会损害细胞的生存能力，因此这种方法可能是生物学家现有技术的独特延伸。
Manipulation of populations of living cells on an individual basis is essential for the investigation of complex interactions among cells. We present a new approach to the integration on silicon of dielectrophoretic actuators and optical sensors that allow; us to carry out this task. The device presented in this paper is an 8 x 8 mm(2) chip implemented in a two-poly three-metal 0.35-mum CMOS technology featuring 102,400 actuation electrodes, arranged in an array of 320 x 320, 20 mum x 20 mum microsites each comprising addressing logic,,an embedded memory for electrode programming, and an optical sensor. The chip enables software-controlled displacement of more than 10,000 individual living cells, allowing biologists to devise complex interaction protocols. that are impossible to manage otherwise. The manipulation does not damage the viability of the cells, so that this approach could be a unique extension to the techniques already available to biologists.