Transient opening of tricellular vertices controls paracellular transport through the follicle epithelium during Drosophila oogenesis
Transient opening of tricellular vertices controls paracellular transport through the follicle epithelium during Drosophila oogenesis
复制标题
DOI：
10.1016/j.devcel.2021.03.021
复制
发表时间：
2021-04-19
影响因子：
11.8
通讯作者：
Luschnig, Stefan
中科院分区：
文献类型：
--
作者：
Isasti-Sanchez, Jone;Munz-Zeise, Fenja;Luschnig, Stefan
调节细胞旁通透性以允许溶质转运或细胞迁移穿过上皮或内皮屏障。然而，细胞-细胞连接动力学如何控制细胞旁通透性知之甚少。在这里，我们描述的通畅性，发育调节的过程中，果蝇卵子发生，在此期间，细胞顶点在滤泡上皮细胞开放短暂，允许卵母细胞吸收的卵黄蛋白的细胞旁运输。我们发现，顺序去除E-钙粘蛋白，N-钙粘蛋白，NCAM/Fasciclin 2，和Sidekick从顶点之前，他们的基底到顶端的开放，而随后的组装三细胞闭塞连接标志着终止通畅性和密封的细胞旁屏障。需要基于E-钙粘蛋白的粘附来限制细胞旁通道大小，而稳定的粘附连接、延长的NCAM/Fasciclin 2表达、阻断的内吞作用或增加的肌动球蛋白收缩性阻止通畅。我们的研究结果揭示了一个关键作用的细胞顶点网关控制细胞旁运输，并表明动态调节的粘附和肌动球蛋白的收缩性顶点支配上皮屏障特性。
Paracellular permeability is regulated to allow solute transport or cell migration across epithelial or endothelial barriers. However, how cell-cell junction dynamics controls paracellular permeability is poorly understood. Here, we describe patency, a developmentally regulated process in Drosophila oogenesis, during which cell vertices in the follicular epithelium open transiently to allow paracellular transport of yolk proteins for uptake by the oocyte. We show that the sequential removal of E-cadherin, N-cadherin, NCAM/Fasciclin 2, and Sidekick from vertices precedes their basal-to-apical opening, while the subsequent assembly of tricellular occluding junctions marks the termination of patency and seals the paracellular barrier. E-cadherinbased adhesion is required to limit paracellular channel size, whereas stabilized adherens junctions, prolonged NCAM/Fasciclin 2 expression, blocked endocytosis, or increased actomyosin contractility prevent patency. Our findings reveal a key role of cell vertices as gateways controlling paracellular transport and demonstrate that dynamic regulation of adhesion and actomyosin contractility at vertices governs epithelial barrier properties.