A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples
A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples
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DOI：
10.1016/j.diagmicrobio.2014.09.004
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发表时间：
2014-12-01
影响因子：
2.9
通讯作者：
Jerome, Keith R.
中科院分区：
文献类型：
--
作者：
Sedlak, Ruth Hall;Kuypers, Jane;Jerome, Keith R.
我们证明了一种用于人巨细胞病毒（CMV）、人腺病毒F种和内部质粒对照的多重液滴数字PCR检测的发展，该检测可能适用于PCR抑制倾向的临床样本。该检测试剂盒在抑制倾向粪便样本中的表现优于CMV定量PCR检测试剂盒，并且是第一个发布的纳入内部对照的临床病毒学液滴数字PCR检测试剂盒。(C)2014爱思唯尔公司All rights reserved.
We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control. (C) 2014 Elsevier Inc. All rights reserved.