The Effect of Cholesterol Feeding and Fasting upon β-Hydroxy-β-methylglutaryl Coenzyme A Reductase
The Effect of Cholesterol Feeding and Fasting upon β-Hydroxy-β-methylglutaryl Coenzyme A Reductase
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进食和禁食胆固醇对β-羟基-β-甲基戊二酰辅酶A还原酶的影响
DOI：
10.1016/s0021-9258(18)96221-6
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发表时间：
1967
影响因子：
4.8
通讯作者：
T. Linn
中科院分区：
文献类型：
--
作者：
T. Linn
在不同饮食条件下饲养的大鼠肝脏制备的无细胞体系中，测定了β-羟基-β-甲基戊二酰辅酶A的酶促还原作用。结果表明，当喂饲胆固醇时，还原酶反应明显受到抑制。当使用丙酮粉末技术时，这种活性在微粒体和随后溶解的制剂中都观察到了减弱。研究了禁食对β-羟基-β-甲基戊二酰辅酶A还原的影响，结果还表明，微粒体中的还原酶活性和可溶性酶中的还原酶活性都显著降低。
The enzymatic reduction of β-hydroxy-β-methylglutaryl coenzyme A to mevalonic acid has been measured in cell-free systems prepared from the livers of rats maintained under various dietary conditions. It has been shown that the reductase reaction is markedly depressed when cholesterol is fed. This diminished activity has been observed in both the microsomes and in the subsequently solubilized preparation when an acetone powder technique is used. The effect of fasting upon β-hydroxy-β-methylglutaryl coenzyme A reduction has been examined and the results also show a dramatic decrease in the reductase activity in both the microsomes and the solubilized enzyme.