Studies on the formation of methylglyoxal from dihydroxyacetone in Manuka (Leptospermum scoparium) honey
Studies on the formation of methylglyoxal from dihydroxyacetone in Manuka (Leptospermum scoparium) honey
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DOI：
10.1016/j.carres.2012.07.025
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发表时间：
2012-11-01
影响因子：
3.1
通讯作者：
Henle, Thomas
中科院分区：
文献类型：
--
作者：
Atrott, Julia;Haberlau, Steffi;Henle, Thomas
二羟基丙酮(DHA)和甲基乙二醛(MGO)是麦卢卡蜂蜜特有的碳水化合物代谢产物。建立了以邻苯二胺(OPD)为衍生化试剂，采用反相高效液相色谱-紫外检测法测定蜂蜜中DHA的方法。DHA与OPD之前未知的反应产物经光谱鉴定为2-羟甲基喹恶啉。对6份直接来自蜂箱的新鲜麦卢卡蜂蜜和18份市售麦卢卡蜂蜜样品进行了DHA的专属测定，范围分别为600~2700 mg/kg和130~1600 mg/kg。新鲜蜂蜜中的氧化镁含量为50~250 mg/kg，市售麦卢卡蜂蜜中的氧化镁含量为70~700 mg/kg。商品蜂蜜中DHA与MGO之间存在良好的线性关系，DHA与MGO的平均比值为2：1。相比之下，新鲜麦冬蜂蜜中DHA与MGO的关系更高，但在蜂蜜成熟时接近2：1。加热实验表明，由于蜂蜜中的焦糖作用，基于热处理的氧化镁的形成不会发生。DHA和MGO可作为麦卢卡蜂蜜适宜的唯一品质参数。(C)2012爱思唯尔有限公司。保留所有权利。
Dihydroxyacetone (DHA) and methylglyoxal (MGO) are unique carbohydrate metabolites of manuka honey. A method for the reliable quantification of DHA in honey samples was established, based on derivatization with o-phenylenediamine (OPD) and subsequent RP-HPLC with UV detection. The previously unknown reaction product of DHA and OPD was identified as 2-hydroxymethylquinoxaline by spectroscopic means. DHA was exclusively determined in 6 fresh manuka honeys originating directly from the beehive as well as 18 commercial manuka honey samples, ranging from 600 to 2700 mg/kg and 130 to 1600 mg/kg, respectively. The corresponding MGO contents varied from 50 to 250 mg/kg in fresh and 70 to 700 mg/kg in commercial manuka honey samples. A good linear correlation between DHA and MGO values in commercial manuka honeys was observed, resulting in a mean ratio of DHA to MGO of 2:1. In contrast to this, the DHA-to-MGO relation was much higher in fresh manuka honeys but approximated to a ratio of 2:1 while honey ripening. Heating experiments revealed that MGO formation based on thermal treatment as a consequence, for example, of caramelization in honey does not occur. DHA and MGO can serve as suitable unique quality parameter for manuka honey. (C) 2012 Elsevier Ltd. All rights reserved.