Molecular identification of a Wolbachia endosymbiont in a Tetranychus urticae strain (Acari: Tetranychidae)
Molecular identification of a Wolbachia endosymbiont in a Tetranychus urticae strain (Acari: Tetranychidae)
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DOI：
10.1111/j.1365-2583.1996.tb00057.x
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发表时间：
1996-08
影响因子：
2.6
通讯作者：
A. Tsagkarakou;T. Gulllemaud;F. Rousset;M. Navajas
中科院分区：
文献类型：
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作者：
A. Tsagkarakou;T. Gulllemaud;F. Rousset;M. Navajas
沃尔巴克氏体是一种母源性细菌，参与节肢动物性行为的改变。在螨虫中经常观察到生殖不相容性，但尚未证明这种微生物在其细胞质中的存在。基于部分 16S rDNA 和 ftsZ 基因的扩增和测序，我们鉴定了二斑叶螨菌株中沃尔巴克氏体的存在。为了建立二斑叶螨中发现的沃尔巴克氏体和其他节肢动物中发现的沃尔巴克氏体之间的系统发育关系，我们将所得序列与已发表的序列进行了比对。对于 16s 和 ftsZ 基因，二斑叶螨携带的沃尔巴克氏体与其他节肢动物中发现的沃尔巴克氏体聚集在一起。
Wolbachia, a maternally lnherlted bacierium, is Involved In alterations of arthropod sexuality. Reproductive incompatlbllities are often observed in mites, but the existence of this microorganism In their cytoplasm has not yet been demonstrated. We Identified the presence of Wolbachia In a strain of the spider mite Tetranychus urticae based on the ampllflcatlon and sequencing of part of the 16S rDNA and ftsZ genes. In order to establish the phylogenetlc relationships between Wolbachia found In T. urticae and In other arthropods, we aligned the resulting sequences with already published ones. For both 16s and ftsZ genes the Wolbachia carried by T. urticae clustered together with Wolbachia found in other arthropods.