Tandem regions of yeast DNA topoisomerase II share homology with different subunits of bacterial gyrase.
Tandem regions of yeast DNA topoisomerase II share homology with different subunits of bacterial gyrase.
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DOI：
10.1126/science.3014661
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发表时间：
1986-08
期刊：
影响因子：
56.9
通讯作者：
R. Lynn;G. Giaever;S. Swanberg;J. Wang
中科院分区：
文献类型：
--
作者：
R. Lynn;G. Giaever;S. Swanberg;J. Wang
将编码 DNA 拓扑异构酶 II 的酿酒酵母基因 TOP2 的核苷酸序列与细菌 DNA 旋转酶的序列进行比较。单亚基酵母酶的氨基和羧基末端半部分分别与细菌促旋酶的 B 和 A 亚基在多肽链上的相应位置显示出同源性。尽管这两种酶在四级结构和活性上都不同，但两种蛋白质之间的同源性表明机械和结构上的相似性以及可能的进化关系。
The nucleotide sequence for the Saccharomyces cerevisiae gene TOP2, which encodes DNA topoisomerase II, was compared with the sequence for bacterial DNA gyrase. The amino and carboxyl terminal halves of the single-subunit yeast enzyme showed homologies with the B and A subunits of bacterial gyrase, respectively, at corresponding positions along the polypeptide chains. Although the two enzymes differ in both quaternary structure and activity, the homology between the two proteins indicates mechanistic as well as structural similarities, and a probable evolutionary relationship.