Phosphoinositide 3-kinase activity is essential for all-trans-retinoic acid-induced granulocytic differentiation of HL-60 cells.
Phosphoinositide 3-kinase activity is essential for all-trans-retinoic acid-induced granulocytic differentiation of HL-60 cells.
复制标题
磷酸肌醇 3-激酶活性对于全反式视黄酸诱导的 HL-60 细胞粒细胞分化至关重要。
作者：
V. Bertagnolo;L. Neri;M. Marchisio;C. Mischiati;S. Capitani
全反式维甲酸诱导HL-60细胞向粒细胞分化后，磷脂酰肌醇3-激酶（PI 3-K）活性增加。通过共聚焦显微镜的免疫化学和免疫细胞化学分析也揭示了酶的量的增加，这在细胞核水平上特别明显。抑制PI 3-K活性的纳摩尔浓度的渥曼青霉素和其表达的转染与p85 α的反义片段阻止了分化过程。所得数据表明PI 3-K活性在促进粒细胞分化中发挥着重要作用。
Phosphoinositide 3-kinase (PI 3-K) activity increases in HL-60 cells that are induced to granulocytic differentiation by all-trans-retinoic acid. Immunochemical and immunocytochemical analyses by confocal microscopy also reveal an increase in the amount of the enzyme, which is particularly evident at the nuclear level. Inhibition of PI 3-K activity by nanomolar concentrations of wortmannin and of its expression by transfection with an antisense fragment of p85alpha prevented the differentiative process. The data obtained indicate that PI 3-K activity plays an essential role in promoting granulocytic differentiation.
大鼠肝核中的磷酸肌醇 3-激酶。
DOI：
10.1021/bi972551g
发表时间：
1998
期刊：
Biochemistry.
影响因子：
--
作者：
Lu,PJ;Hsu,AL;Wang,DS;Yan,HY;Yin,HL;Chen,CS
通讯作者：
Chen,CS
DOI：
10.1016/s0021-9258(18)41950-3
发表时间：
1992-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Soltoff;S. Rabin;L. Cantley;D. Kaplan
通讯作者：
S. Soltoff;S. Rabin;L. Cantley;D. Kaplan
DMSO 诱导分化过程中鼠红白血病细胞中磷脂酰肌醇 3-激酶的活性。
DOI：
10.1006/excr.1995.1252
发表时间：
1995
期刊：
Experimental cell research.
影响因子：
--
作者：
Ai,Z;Misra,S;Susa,M;Varticovski,L;Cohen,CM
通讯作者：
Cohen,CM