High-speed micellar electrokinetic capillary chromatography of the common phosphorylated nucleosides
High-speed micellar electrokinetic capillary chromatography of the common phosphorylated nucleosides
复制标题
常见磷酸化核苷的高速胶束电动毛细管色谱法
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
M. Novotny
中科院分区：
文献类型：
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作者：
Jinping Liu;J. Banks;M. Novotny
本文研究了胶束电动毛细管色谱（MECC）快速分离单磷酸化、二磷酸化和三磷酸化核糖核苷的方法。电渗流、峰容量（to/tmc）和分离度方面的保留行为可以通过选择表面活性剂类型和缓冲相中的浓度来控制。的阴离子表面活性剂，SDS，和两个阳离子表面活性剂，DTAB和HTAB，的相对影响进行了探索和优化，以产生一个有效的分离的溶质。
A high-speed separation of the mono-, di-, and triphosphorylated common ribonucleosides has been explored using micellar electrokinetic capillary chromatography (MECC). Retention behavior in terms of electroosmotic flow, peak capacity (to/tmc) and resolution can be manipulated by the choice of surfactant type and the concentration in the buffer phase. The relative effects of an anionic surfactant, SDS, and two cationic surfactants, DTAB and HTAB, were explored and optimized to produce an effective separation of the solutes.