Cell-mediated destruction of human leukemic cells by MHC identical lymphocytes: requirement for a proliferative trigger in vitro.
Cell-mediated destruction of human leukemic cells by MHC identical lymphocytes: requirement for a proliferative trigger in vitro.
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MHC 相同淋巴细胞对人类白血病细胞的细胞介导破坏：体外增殖触发的要求。
DOI：
10.4049/jimmunol.117.6.2197
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发表时间：
1976
影响因子：
4.4
通讯作者：
L. Chess
中科院分区：
文献类型：
--
作者：
P. Sondel;C. O'brien;L. Porter;S. Schlossman;L. Chess
这些实验已经研究了细胞机制参与产生细胞免疫应答人类急性白血病细胞。由于正常的人类淋巴细胞在体外不能从免疫上识别来自MHC相同的兄弟姐妹的淋巴细胞，目前的研究已经检查了正常淋巴细胞对MHC相同的AML和ALL细胞的体外增殖和细胞毒性反应。在那些急性白血病细胞不能通过MHC相同的淋巴细胞诱导增殖反应的情况下，产生有效的抗白血病细胞毒性需要在致敏培养中添加不相关的刺激细胞。相反，由MHC相同淋巴细胞诱导增殖反应的白血病母细胞也能够刺激抗白血病细胞毒性。这可以通过在致敏培养物中添加不相关的刺激细胞来增强。通过对51Cr白血病母细胞以及51Cr标记的MHC相同的植物血凝素母细胞或正常淋巴细胞同时进行假定杀伤细胞的测试，证明了抗白血病细胞细胞毒性的特异性。同时致敏MHC相同的白血病母细胞和不相关的刺激淋巴细胞并不一定产生抗白血病细胞毒性，即使观察到异体细胞毒性；在这些非反应性组合中缺乏可证实的抑制活性表明，一些个体可能具有特异性免疫功能不全，因此无法产生有效的抗白血病CML。
These experiments have investigated cellular mechanisms involved in the generation of cellular immune responses to human acute leukemic blasts. Because normal human lymphocytes are not able to recognize immunologically, in vitro, lymphocytes from MHC identical siblings, the present studies have examined the in vitro proliferative and cytotoxic responses of normal lymphocytes to MHC identical AML and ALL blasts. In those cases where acute leukemic cells were unable to induce a proliferative response by MHC identical lymphocytes, the generation of effective anti-leukemic cytotoxicity required the addition of unrelated stimulating cells to the sensitization culture. In contrast, leukemic blasts that induced a proliferative response by MHC identical lymphocytes were also able to stimulate anti-leukemic cytotoxicity. This could be augmented by the addition of unrelated stimulating cells to the sensitization culture. The specificity of anti-leukemic cell cytotoxicity was demonstrated in all instances by simultaneous testing of putative killer cells on 51Cr leukemic blasts as well as 51Cr-labeled MHC identical phytohemagglutinin blasts or normal lymphocytes. Simultaneous sensitization to MHC identical leukemic blasts and unrelated stimulating lymphocytes did not invariably generate anti-leukemic cytotoxicity even when allogeneic cytotoxicity was observed; the absence of demonstrable suppressor activity in these nonreactive combinations suggested that some individuals may be specifically immunoincompetent, and thereby unable to generate effective anti-leukemic CML.