Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
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DOI：
10.1073/pnas.0832308100
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发表时间：
2003-06-10
影响因子：
11.1
通讯作者：
Webb, WW
中科院分区：
文献类型：
--
作者：
Zipfel, WR;Williams, RM;Webb, WW
使用双光子和三光子激发的本征荧光结合超分子结构的二次谐波产生的活组织多色非线性显微镜产生的图像具有标准组织学的分辨率和细节，而无需使用外源染色。组织内固有指标的成像，如烟酰胺腺嘌呤二核苷酸、视黄醇、吲哚胺和胶原蛋白，为生理和病理提供了重要信息。有效地应用多光子显微镜的内在成像需要特定的细胞和组织成分的非线性光学特性的知识。在这里，我们编译并演示了涉及一系列内在分子和分子组装的应用，这些应用可以直接可视化组织形态、细胞代谢和疾病状态，如阿尔茨海默病和癌症。
Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, in-doleamines, and collagen provides crucial information for physiology and pathology. The efficient application of multiphoton microscopy to intrinsic imaging requires knowledge of the nonlinear optical properties of specific cell and tissue components. Here we compile and demonstrate applications involving a range of intrinsic molecules and molecular assemblies that enable direct visualization of tissue morphology, cell metabolism, and disease states such as Alzheimer's disease and cancer.