Combining the Dynamic TNO-Gastrointestinal Tract System with a Caco-2 Cell Culture Model: Application to the Assessment of Lycopene and α-Tocopherol Bioavailability from a Whole Food
Combining the Dynamic TNO-Gastrointestinal Tract System with a Caco-2 Cell Culture Model: Application to the Assessment of Lycopene and α-Tocopherol Bioavailability from a Whole Food
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DOI：
10.1021/jf902392a
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发表时间：
2009-12-09
影响因子：
6.1
通讯作者：
Alric, Monique
中科院分区：
文献类型：
--
作者：
Deat, Emmanuelle;Blanquet-Diot, Stephanie;Alric, Monique
为了发挥它们的健康作用，植物化学物质如类胡萝卜素和维生素E必须是生物可利用的。本研究首次采用Caco-2细胞耦合的动态胃肠系统TNO胃肠道模型（TIM）研究了含红番茄和葵花籽油的全食物中番茄红素和α -生育酚的消化稳定性和肠道吸收。消化样品经适当的超离心、过滤和稀释后加入Caco-2细胞。在TIM中，α -生育酚在消化过程中是稳定的，而番茄红素则损失了25%。两种化合物的吸收均呈曲线、双向和浓度依赖性。消化系统吸收α -生育酚的百分比比纯化合物低，但番茄红素没有，这表明与测试餐中的其他成分竞争吸收。根据体内数据，发现番茄红素的生物利用度比α -生育酚低。这些结果支持这种体外方法用于估计食品中活性化合物的生物利用度的有效性。
To exert their health effect, phytochemicals such as carotenoids and vitamin E have to be bioavailable. We investigated the digestive stability and intestinal absorption of lycopene and alpha-tocopherol from a whole food containing red tomatoes and sunflower oil using, for the first time, the dynamic gastrointestinal system TNO gastrointestinal tract model (TIM) coupled with Caco-2 cells. Digestive samples were added to Caco-2 cells after appropriate ultracentrifugation, filtration, and dilution. alpha-Tocopherol was stable during digestion in the TIM, whereas a 25% loss was observed for lycopene. The absorption of both compounds was curvilinear, bidirectional, and concentration-dependent. The percentages of alpha-tocopherol absorbed, but not that of lycopene, were lower with digestas compared to those with pure compounds, suggesting competition for absorption with other components of the test meal. According to in vivo data, a lower bioavailability was found for lycopene compared to that for alpha-tocopherol. These results support the usefulness of this in vitro approach for estimating the bioavailability of active compounds from food.