THE EFFECT OF DIGESTION WITH STREPTOMYCES HYALURONIDASE UPON CERTAIN HISTOCHEMICAL REACTIONS OF HYALURONIC ACID-CONTAINING TISSUES

THE EFFECT OF DIGESTION WITH STREPTOMYCES HYALURONIDASE UPON CERTAIN HISTOCHEMICAL REACTIONS OF HYALURONIC ACID-CONTAINING TISSUES
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链霉菌透明质酸酶消化对含透明质酸组织的某些组织化学反应的影响

DOI:
10.1177/21.9.794
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发表时间:
1973
影响因子:
3.2
通讯作者:
K. Yamada
K. Yamada
中科院分区:
生物学3区
文献类型:
--
作者:
K. Yamada

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在一系列人、哺乳动物和禽类标本上,研究了链霉菌透明质酸酶消化对含透明质酸组织某些组织化学反应的影响。这些组织化学反应是为了展示中性的、硫酸盐化的和非硫酸盐化的酸性粘液糖,如高铁希夫、阿尔新蓝(pH 1.0)、天青A(pH 1.5)、高铁二胺、醛品红、阿尔新蓝(pH 2.5)、阿尔新蓝(pH 2.5)-高碘酸希夫、天青A(pH 4.5)、低铁二胺和胶体铁。此外,还观察了睾丸透明质酸酶消化对同一组织相同反应的影响,以供比较。用链霉菌透明质酸酶消化可以减少透明质酸引起的染色反应,但不会影响中性和硫酸盐化的酸性粘多糖的染色反应。这些结果表明,在粘多糖组织化学中,用链霉菌透明质酸酶消化法鉴定透明质酸是一种较好的方法。
The effect of digestion with Streptomyces hyaluronidase upon certain histochemical reactions of hyaluronic acid-containing tissues has been studied in a series of human, mammalian and avian specimens. These histochemical reactions are those for the demonstration of neutral and sulfated and nonsulfated acid mucosaccharides such as periodic acid-Schiff, Alcian Blue (pH 1.0), azure A (pH 1.5), high iron diamine, aldehyde fuchsin, Alcian Blue (pH 2.5), Alcian Blue (pH 2.5)-periodic acid Schiff, azure A (pH 4.5), low iron diamine and colloidal iron. In addition, the effect of digestion with testicular hyaluronidase upon the same reactions of the same tissues was observed for comparison. Digestion with Streptomyces hyaluronidase diminishes the staining reactions due to hyaluronic acid but fails to affect those for neutral and sulfated acid mucosaccharides. These results indicate that digestion with Streptomyces hyaluronidase is a method of choice for the identification of hyaluronic acid in mucosaccharide histochemistry.
Watanabe, T. 等人:“大鼠肝细胞中血管紧张素原和组织蛋白酶 B、H 和 L 的免疫细胞化学定位,特别是秋水仙碱后血管紧张素原的降解。”
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