Senescent jejunal mast cells and eosinophils in the mouse preferentially translocate to the spleen and draining lymph node, respectively, during the recovery phase of helminth infection
Senescent jejunal mast cells and eosinophils in the mouse preferentially translocate to the spleen and draining lymph node, respectively, during the recovery phase of helminth infection
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DOI：
10.4049/jimmunol.165.1.344
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发表时间：
2000-07-01
影响因子：
4.4
通讯作者：
Stevens, RL
中科院分区：
文献类型：
--
作者：
Friend, DS;Gurish, MF;Stevens, RL
由于感染旋毛虫的小鼠肠道内会出现明显但短暂的肥大细胞增多症和嗜酸性粒细胞增多，因此该疾病模型被用来追踪衰老 T 细胞依赖性肥大细胞 (MC) 和嗜酸性粒细胞的命运。在感染消退阶段，空肠中发现很少有发生凋亡的 MC 或嗜酸性粒细胞，尽管凋亡 MC 在大肠中很常见。尽管肠系膜引流淋巴结含有大量凋亡的嗜酸性粒细胞，但在该位置很少发现MC。在恢复阶段，脾脏中存在大量 MC，并且其中许多细胞具有分段的细胞核。这些脾脏 MC 没有增殖。尽管来自未感染小鼠的空肠和脾脏的 MC 未能表达小鼠 MC 蛋白酶 (mMCP) 9，但基本上 T，spiralis 感染小鼠的空肠粘膜下层和脾中的所有 MC 在恢复阶段都表达了这种丝氨酸蛋白酶。在脾脏中检测到任何含有 mMCP-9 的细胞之前，空肠中的 MC 就表达了 mMCP-9。当这些细胞开始从空肠消失时，在脾血管中检测到含有 mMCP-9 的 MC，这一事实支持了许多空肠 MC 在感染恢复阶段易位至脾的观点。在此易位过程中，一些衰老的空肠 MC 会发生核分割。这些研究首次揭示了蠕虫感染期间 T 细胞依赖性嗜酸性粒细胞和 MC 在空肠中扩张后的不同退出和处理途径。
Because mice infected with Trichinella spiralis experience a pronounced, but transient, mastocytosis and eosinophilia in their intestine, this disease model was used to follow the fate of senescent T cell-dependent mast cells (MCs) and eosinophils. Very few MCs or eosinophils undergoing apoptosis were found in the jejunum during the resolution phase of the infection, even though apoptotic MCs were common in the large intestine. Although the mesenteric draining lymph nodes contained large numbers of apoptotic eosinophils, MCs were rarely found at this location. During the recovery phase, large numbers of MCs were present in the spleen, and many of these cells possessed segmented nuclei. These splenic MCs were not proliferating. Although MCs from the jejunum and spleen of noninfected mice failed to express mouse MC protease (mMCP) 9, essentially all of the MCs in the jejunal submucosa and spleen of T, spiralis-infected mice expressed this serine protease during the recovery phase. The MCs in the jejunum expressed mMCP-9 before any mMCP-9-containing cells could be detected in the spleen. The fact that mMCP-9-containing MCs were detected in splenic blood vessels as these cells began to disappear from the jejunum supports the view that many jejunal MCs translocate to the spleen during the recovery phase of the infection. During this translocation process, some senescent jejunal MCs undergo nuclear segmentation. These studies reveal for the first time different exit and disposal pathways for T cell-dependent eosinophils and MCs after their expansion in the jejunum during a helminth infection.