Functional characteristics of neutrophils collected and stored after administration of G-CSF
Functional characteristics of neutrophils collected and stored after administration of G-CSF
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DOI：
10.1046/j.1537-2995.2000.40040414.x
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发表时间：
2000-04-01
期刊：
影响因子：
2.9
通讯作者：
Ambruso, DR
中科院分区：
文献类型：
--
作者：
Leavey, PJ;Thurman, G;Ambruso, DR
背景：粒细胞输注可用于患有严重细菌或真菌感染且对抗生素治疗无反应的中性粒细胞减少患者。然而，无法储存粒细胞浓缩物限制了它们的临床用途。 研究设计和方法：在单采收集后和储存至 48 小时期间检查中性粒细胞趋化性和 NADPH 氧化酶活性以及中性粒细胞 NADPH 氧化酶系统的完整性：中性粒细胞通过 G-CSF 在体内动员，通过单采技术收集，并在存在或不存在额外成分的情况下储存在单采袋中G-CSF。对于所有实验，细胞均通过葡聚糖沉降和低渗红细胞裂解的标准技术进一步纯化。结果：中性粒细胞趋化性在储存24小时内得以保留，但不受添加到储存单元中的G-CSF的影响。 NADPH 氧化酶系统也作为功能复合物得以保留，胞质蛋白和膜相关蛋白在 48 小时内均保持正常。然而，完整细胞对无细胞系统中的激活刺激和氧化酶活性降低有不同的反应。 G-CSF 在储存过程中似乎没有显着影响 NADPH 氧化酶活性或 NADPH 氧化酶系统完整性。结论：给予 G-CSF 后收集的中性粒细胞在储存至少 24 小时内保留了功能和生化特征，这表明 G-CSF 动员除了提高 PMN 产量和收集后储存这些成分的可能性之外还有其他作用。
BACKGROUND: Granulocyte transfusion may be used in neutropenic patients with severe bacterial or fungal infections that are unresponsive to antibiotic therapy. However, the inability to store granulocyte concentrates limits their clinical usefulness.STUDY DESIGN AND METHODS: Neutrophil chemotaxis and NADPH oxidase activity and the integrity of the neutrophil NADPH oxidase system were examined after apheresis collection and during storage to 48 hours: Neutrophils were mobilized in vivo by G-CSF, collected by apheresis techniques, and stored in apheresis bags in the presence and absence of additional G-CSF. For all experiments, cells were further purified by standard techniques of dextran sedimentation and hypotonic RBC lysis.RESULTS: Neutrophil chemotaxis was preserved to 24 hours of storage but was not affected by the G-CSF added to storage units. The NADPH oxidase system was also preserved as a functioning complex, and both cytosolic protein's and membrane-associated proteins were normal to 48 hours. However, there were divergent responses by intact cells to activating stimuli and reduced oxidase activity in the cell-free system. G-CSF did not appear to significantly affect NADPH oxidase activity or NADPH oxidase system integrity during storage.CONCLUSION: Neutrophils collected after the administration of G-CSF retained functional and biochemical characteristics for at least 24 hours of storage, which suggests additional effects of G-CSF mobilization beyond enhancing PMN yields and the possibility of storage of these components after collection.