Differential Mobility Separation of Leukotrienes and Protectins
Differential Mobility Separation of Leukotrienes and Protectins
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DOI：
10.1021/acs.analchem.5b00786
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发表时间：
2015-05-19
影响因子：
7.4
通讯作者：
Giera, Martin
中科院分区：
文献类型：
--
作者：
Jonasdottir, Hulda S.;Papan, Cyrus;Giera, Martin
差分迁移谱 (DMS) 能够根据立体异构分子离子在具有不对称波形的振荡电场中的迁移率来分离立体异构分子离子。因此，它是色谱法和（串联）质谱法的“正交”技术。生物活性脂质，特别是类二十烷酸和二十二烷酸类，具有多种立体异构体，表现出高度特异性的结构、活性关系。此外，这些化合物的几何形状也反映了它们的生化起源。因此，类二十烷酸和二十二烷酸类相关异构体的明确表征对于理解它们在许多生物过程中的起源和功能至关重要：在这里，我们表明，SelexION DMS 技术与 mu LC-MS/MS 联用，能够区分至少五种密切相关的白三烯，这些白三烯部分共洗脱并且仅使用 LC-MS/MS 无法解析。我们将开发的方法应用于小鼠腹膜渗出液细胞中的 LTB4 及其共洗脱异构体 5S,12S-diHETE 的分离，结果表明 LTB4 仅在注射酵母聚糖 A 后才存在，而其异构体 5S,12S-diHETE 在施用盐水 (PBS) 后产生。此外，我们还表明 SelexION 技术还可应用于两种异构保护素 Pal 和 PDX (10S,17S-diHDHA) 的分离。
Differential mobility spectrometry (DMS) is capable of separating stereoisomeric molecular ions based on their mobility in an oscillating electrical field with an asymmetric waveform. Thus, it is an "orthogonal" technique to chromatography and (tandem) mass spectrometry. Bioactive lipids, particularly of the eicosanoid and docosanoid class feature numerous stereoisomers, which exhibit a highly specific structure, activity relationship. Moreover, the geometry of these compounds also reflects their, biochemical origin. Therefore, the unambiguous characterization of related isomers of the eicosanoid and docosanoid classes is of fundamental importance to the understanding of their origin: and function, in many biological processes: Here we show, That SelexION DMS technology coupled to mu LC-MS/MS is,capable of differentiating at least five closely related leukotrienes partially coeluting and (almost) unresolvable using LC-MS/MS only. We applied the developed method, to the separation of LTB4 and its coeluting isomer 5S,12S-diHETE in murine peritoneal exudate cells, showing that LTB4 is present only after zymosan A injection while its isomer 5S,12S-diHETE is produced after saline, (PBS) administration. Additionally, we show that the SelexION technology can also be applied to the separation of Pal and PDX (10S,17S-diHDHA), two isomeric protectins.