Quantitation of varicella-zoster virus DNA in patients with Ramsay Hunt syndrome and zoster sine herpete.
Quantitation of varicella-zoster virus DNA in patients with Ramsay Hunt syndrome and zoster sine herpete.
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拉姆齐亨特综合征和正弦疱疹患者的水痘-带状疱疹病毒 DNA 定量。
DOI：
10.1097/00129492-200200001-00091
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发表时间：
2001
影响因子：
9.4
通讯作者：
Yukio Inuyama
中科院分区：
文献类型：
--
作者：
Yasushi Furuta;F. Ohtani;Hiroshi Aizawa;Satoshi Fukuda;Yukio Inuyama
水痘-带状疱疹病毒（VZV）再激活分别导致Ramsay Hunt综合征（RHS）和无带状疱疹性带状疱疹（ZBH）伴和不伴带状疱疹性皮疹的面神经麻痹。在本研究中，我们分析了VZV的DNA拷贝数唾液样本从25例RHS和31例SAH患者使用TaqMan PCR检测，以确定两种疾病之间的病毒载量的差异。唾液中的VZV拷贝数在RHS患者带状疱疹出现的当天附近达到峰值。因此，在带状疱疹出现几天后表现出面瘫的RHS患者中，VZV DNA的检出率较低。这些发现表明，唾液样本中的VZV载量反映了RHS患者中病毒再活化的动力学。此外，VZV DNA在RHS和SAH患者的唾液中同样检测到，并且RHS和SAH患者之间的最高病毒拷贝数没有显著差异。VZV负载似乎并不反映RHS和CHTH之间的主要差异。
Varicella-zoster virus (VZV) reactivation causes facial nerve palsy in Ramsay Hunt syndrome (RHS) and zoster sine herpete (ZSH) with and without zoster rash, respectively. In the present study, we analyzed the VZV DNA copy number in saliva samples from 25 patients with RHS and 31 patients with ZSH using a TaqMan PCR assay to determine differences in the viral load between the two diseases. VZV copy number in saliva peaked near the day of the appearance of zoster in patients with RHS. Consequently, VZV DNA was less frequently detected in patients with RHS who exhibited facial palsy several days after the appearance of zoster. These findings suggest that the VZV load in saliva samples reflects the kinetics of viral reactivation in patients with RHS. In addition, VZV DNA was equally detected in saliva from patients with RHS and ZSH, and there was no significant difference in the highest viral copy number between patients with RHS and those with ZSH. The VZV load does not appear to reflect a major difference between RHS and ZSH.