Extrinsic mechanical forces mediate retrograde axon extension in a developing neuronal circuit.
Extrinsic mechanical forces mediate retrograde axon extension in a developing neuronal circuit.
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DOI：
10.1038/s41467-017-00283-3
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发表时间：
2017-08-17
影响因子：
16.6
通讯作者：
Schneider-Maunoury S
中科院分区：
文献类型：
--
作者：
Breau MA;Bonnet I;Stoufflet J;Xie J;De Castro S;Schneider-Maunoury S
为了形成功能性神经回路，神经元迁移到它们的最终目的地，并将轴突延伸到它们的目标。这两个过程是否以及如何在体内协调仍然是难以捉摸的。我们使用斑马鱼的嗅觉基板作为一个系统，以解决潜在的机制。定量实时成像揭示了一个舞蹈的定向细胞运动的形状基板神经元簇：收敛的细胞向基板域的中心和侧向细胞运动远离大脑。轴突形成伴随着侧向运动，并通过一种意想不到的逆行延伸模式发生，其中细胞体远离附着在脑表面的轴突尖端。会聚运动是主动的，而细胞体侧向位移主要是被动的，可能是由会聚相邻细胞的压缩力触发的。这些发现揭示了神经元回路形成的一种以前未知的机制，即外在机械力驱动轴突的逆行延伸。神经元迁移和轴突生长在发育过程中如何协调只被部分理解。在这里，作者使用定量成像来描述斑马鱼嗅觉基板的形态发生，并报告了一个意想不到的现象，即轴突通过神经元细胞体的被动运动远离拴系的轴突尖端延伸。
To form functional neural circuits, neurons migrate to their final destination and extend axons towards their targets. Whether and how these two processes are coordinated in vivo remains elusive. We use the zebrafish olfactory placode as a system to address the underlying mechanisms. Quantitative live imaging uncovers a choreography of directed cell movements that shapes the placode neuronal cluster: convergence of cells towards the centre of the placodal domain and lateral cell movements away from the brain. Axon formation is concomitant with lateral movements and occurs through an unexpected, retrograde mode of extension, where cell bodies move away from axon tips attached to the brain surface. Convergence movements are active, whereas cell body lateral displacements are of mainly passive nature, likely triggered by compression forces from converging neighbouring cells. These findings unravel a previously unknown mechanism of neuronal circuit formation, whereby extrinsic mechanical forces drive the retrograde extension of axons. How neuronal migration and axon growth coordinate during development is only partially understood. Here the authors use quantitative imaging to characterise the morphogenesis of the zebrafish olfactory placode and report an unexpected phenomenon, whereby axons extend through the passive movement of neuron cell bodies away from tethered axon tips.
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影响因子：
2.7
作者：
Breau, Marie Anne;Schneider-Maunoury, Sylvie
通讯作者：
Schneider-Maunoury, Sylvie
影响因子：
16.2
作者：
Jacobson, C;Schnapp, B;Banker, GA
通讯作者：
Banker, GA
影响因子：
56.9
作者：
Behrndt, Martin;Salbreux, Guillaume;Heisenberg, Carl-Philipp
通讯作者：
Heisenberg, Carl-Philipp
DOI：
10.1523/jneurosci.4442-11.2012
发表时间：
2012-03-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lakhina V;Marcaccio CL;Shao X;Lush ME;Jain RA;Fujimoto E;Bonkowsky JL;Granato M;Raper JA
通讯作者：
Raper JA
影响因子：
21.3
作者：
Collinet, Claudio;Rauzi, Matteo;Lecuit, Thomas
通讯作者：
Lecuit, Thomas