Compact fiber-based multi-photon endoscope working at 1700 nm
Compact fiber-based multi-photon endoscope working at 1700 nm
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DOI：
10.1364/boe.9.002326
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发表时间：
2018-05-01
影响因子：
3.4
通讯作者：
Kieu, Khanh
中科院分区：
文献类型：
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作者：
Akhoundi, Farhad;Qin, Yukun;Kieu, Khanh
我们提出了一种基于压电扫描管的紧凑型多光子内窥镜的设计、实现和性能分析。设计了一种具有长工作距离和高数值孔径（约0.5）的微型物镜透镜，以提供衍射极限光斑尺寸。此外，使用1700 nm波长的飞秒光纤激光器作为激发源，以克服生物组织的散射，减少水的吸收。因此，新颖的光学系统沿着独特的波长使我们能够增加成像深度。我们证明，内窥镜是能够执行第三次和第二次谐波产生（THG/SHG）和三光子激发荧光（3 PEF）成像在一个大的视野（> 400 μ m）与高横向分辨率（2.2 μ m）。紧凑和轻便的探头设计使其适合微创体内成像，作为手术活检的潜在替代方案。(C)根据OSA开放获取出版协议的条款，2018年美国光学学会
We present the design, implementation and performance analysis of a compact multi-photon endoscope based on a piezo electric scanning tube. A miniature objective lens with a long working distance and a high numerical aperture (approximate to 0.5) is designed to provide a diffraction limited spot size. Furthermore, a 1700 nm wavelength femtosecond fiber laser is used as an excitation source to overcome the scattering of biological tissues and reduce water absorption. Therefore, the novel optical system along with the unique wavelength allows us to increase the imaging depth. We demonstrate that the endoscope is capable of performing third and second harmonic generation (THG/SHG) and three-photon excitation fluorescence (3PEF) imaging over a large field of view (> 400 mu m) with high lateral resolution (2.2 mu m). The compact and lightweight probe design makes it suitable for minimally-invasive in-vivo imaging as a potential alternative to surgical biopsies. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement