Communication - Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase
Communication - Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase
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DOI：
10.1074/jbc.272.45.28194
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发表时间：
1997-11-07
影响因子：
4.8
通讯作者：
Sung, P
中科院分区：
文献类型：
--
作者：
Sung, P
酿酒酵母的RAD 51和RAD 52基因是遗传重组和DNA双链断裂修复所需的RAD 52上位性组的关键成员。RAD51编码的产物介导DNA链交换反应。有效的链交换取决于在Rad51已成核到单链DNA上之后添加异源三聚体单链DNA结合因子复制蛋白A（RPA）。然而，如果将单链DNA与Rad51和RPA同时孵育以模拟可能预期在体内发生的情况，则链交换的效率显著降低，揭示了RPA的抑制作用不同于其刺激功能。有趣的是，包含Rad52蛋白，其已在本研究中从酵母细胞中纯化，恢复了链交换的效率。因此，Rad52作为Rad51重组酶的辅因子发挥作用，特异性地发挥作用以克服RPA与单链DNA结合的明显竞争。
The RAD51 and RAD52 genes of Saccharomyces cerevisiae are key members of the RAD52 epistasis group required for genetic recombination and the repair of DNA double-stranded breaks. The RAD51 encoded product mediates the DNA strand exchange reaction. Efficient strand exchange is contingent upon the addition of the heterotrimeric single-stranded DNA binding factor replication protein A (RPA) after Rad51 has nucleated onto the single-stranded DNA However, if the single-stranded DNA is incubated with Rad51 and RPA simultaneously to mimic what may be expected to occur in vivo, the efficiency of strand exchange decreases dramatically, revealing an inhibitory effect of RPA that is distinct from its stimulatory function. Interestingly, the inclusion of Rad52 protein, which has been purified in this study from yeast cells, restores the efficiency of strand exchange. Thus, Rad52 functions as a co-factor for the Rad51 recombinase, acting specifically to overcome the apparent competition by RPA for binding to single-stranded DNA.