Two Distinct L-Lactate Dehydrogenases Play a Role in the Survival of Neisseria gonorrhoeae in Cervical Epithelial Cells
Two Distinct L-Lactate Dehydrogenases Play a Role in the Survival of Neisseria gonorrhoeae in Cervical Epithelial Cells
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DOI：
10.1093/infdis/jiz468
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发表时间：
2020-02-01
影响因子：
6.4
通讯作者：
McEwan, Alastair G.
中科院分区：
文献类型：
--
作者：
Chen, Nathan H.;Ong, Cheryl-lynn Y.;McEwan, Alastair G.
L-乳酸盐是宿主生物体中大量小生境中的代谢产物，并且是细菌病原体如淋病奈瑟氏菌的重要碳源。在这项研究中，我们描述了一种替代，铁硫簇含L-乳酸脱氢酶（LutACB），这是从黄素蛋白L-乳酸脱氢酶（LldD）不同。lutACB的表达被发现是正调控铁，而lldD更高的表达条件下的铁限制。LutACB和LldD的功能作用反映在生长的体外研究中，并在原代宫颈上皮细胞中的淋病奈瑟菌的生存。
L-lactate is an abundant metabolite in a number of niches in host organisms and represents an important carbon source for bacterial pathogens such as Neisseria gonorrhoeae. In this study, we describe an alternative, iron-sulfur cluster-containing L-lactate dehydrogenase (LutACB), that is distinct from the flavoprotein L-lactate dehydrogenase (LldD). Expression of lutACB was found to be positively regulated by iron, whereas lldD was more highly expressed under conditions of iron-limitation. The functional role of LutACB and LldD was reflected in in vitro studies of growth and in the survival of N gonorrhoeae in primary cervical epithelial cells.