Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto.
Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto.
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严格伯氏疏螺旋体当地群体中 ospC 的遗传多样性。
DOI：
10.1093/genetics/151.1.15
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Luft,BJ
中科院分区：
文献类型：
--
作者：
Wang,IN;Dykhuizen,DE;Qiu,W;Dunn,JJ;Bosler,EM;Luft,BJ
严格伯氏疏螺旋体（莱姆病的病原体）的外表面蛋白 OspC 高度可变。我们已经证明，即使在单一群体中，OspC 也具有很大的可变性。使用 PCR-SSCP 测定了从纽约谢尔特岛的一个地点收集的 40 只受感染的鹿蜱中的 ospA 和 ospC 变异。 ospA 和 ospC 等位基因之间存在非常强烈的明显连锁不平衡，即使它们位于不同的质粒上。确定了 ospC 的 13 个可辨别的 SSCP 迁移类别，并确定了每个类别的 DNA 序列。这些序列与 40 个 GenBank 序列相结合，使我们能够定义 19 个主要的 ospC 组。主要 ospC 组内的序列彼此之间平均差异 <1%，而主要 ospC 组之间的序列平均差异约 20%。蜱样本包含 11 个主要的 ospC 组，GenBank 包含 16 个组，两个样本中都发现了 8 个组。因此，当地种群内的 ospC 变异几乎与整个物种的类似大小样本的变异一样大。等位基因频率的 Ewens-Watterson-Slatkin 检验显示与中性期望存在显着偏差，表明这些主要 ospC 群体的平衡选择。如果要将 OspC 蛋白用作血清诊断抗原或疫苗，则需要考虑主要 ospC 基团所代表的变异。
The outer surface protein, OspC, is highly variable in Borrelia burgdorferi sensu stricto, the agent of Lyme disease. We have shown that even within a single population OspC is highly variable. The variation of ospA and ospC in the 40 infected deer ticks collected from a single site on Shelter Island, New York, was determined using PCR-SSCP. There is very strong apparent linkage disequilibrium between ospA and ospC alleles, even though they are located on separate plasmids. Thirteen discernible SSCP mobility classes for ospC were identified and the DNA sequence for each was determined. These sequences, combined with 40 GenBank sequences, allow us to define 19 major ospC groups. Sequences within a major ospC group are, on average, <1% different from each other, while sequences between major ospC groups are, on average, ∼20% different. The tick sample contains 11 major ospC groups, GenBank contains 16 groups, with 8 groups found in both samples. Thus, the ospC variation within a local population is almost as great as the variation of a similar-sized sample of the entire species. The Ewens-Watterson-Slatkin test of allele frequency showed significant deviation from the neutral expectation, indicating balancing selection for these major ospC groups. The variation represented by major ospC groups needs to be considered if the OspC protein is to be used as a serodiagnostic antigen or a vaccine.