Phosphorylation of histone H2A is associated with centromere function and maintenance in meiosis
Phosphorylation of histone H2A is associated with centromere function and maintenance in meiosis
复制标题
组蛋白 H2A 的磷酸化与减数分裂中着丝粒的功能和维持相关
DOI：
10.1111/j.1365-313x.2012.05029.x
复制
发表时间：
2012-09-01
期刊：
影响因子：
7.2
通讯作者：
Han, Fangpu
中科院分区：
文献类型：
--
作者：
Dong, Qianhua;Han, Fangpu
组蛋白磷酸化在细胞分裂过程中受到动态调节，例如组蛋白 H3 (H3)-Ser10、H3-Thr11 和 H3-Ser28 的磷酸化。在这里，我们分析了玉米 (Zea mays L) 的 Thr133 磷酸化组蛋白 H2A，这对于哺乳动物和酵母中纺锤体检查点控制和着丝粒粘合保护器 Shugoshin 的定位非常重要。免疫染色结果表明，通过使用显示黄色荧光蛋白-CENH3信号的植物，磷酸化的H2A-Thr133信号桥接了着丝粒H3组蛋白变体CENH3的信号，并且H2A-Thr133在不同细胞类型中被磷酸化。在有丝分裂期间，H2A-Thr133 磷酸化在中期变得强烈，并且是着丝粒区域特异的，但在后期和末期下降。对几个玉米双着丝粒染色体的免疫染色显示，失活的着丝粒已经失去了 H2A-Thr133 的磷酸化。在玉米减数分裂过程中，H2A磷酸化在粗线期早期变得很强，并在中期I增加到最大。在玉米减数分裂突变体afd1（没有第一次分裂）中，姐妹染色单体在中期I表现出等式分离，但与野生型相比，减数分裂期间H2A-Thr-133磷酸化没有变化。在 sgo1 突变体中，姐妹染色单体在减数分裂 II 期间随机分离，并且在减数分裂 II 期间在着丝粒区域观察到 H2A-Thr-133 的磷酸化。玉米中缺乏姐妹内聚力和 Shugoshin 的此类突变体的可用性表明，磷酸化信号不依赖于内聚力，而是依赖于着丝粒活性。
Histone phosphorylation is dynamically regulated during cell division, for example phosphorylation of histone H3 (H3)-Ser10, H3-Thr11 and H3-Ser28. Here we analyzed maize (Zea mays L) for Thr133-phosphorylated histone H2A, which is important for spindle checkpoint control and localization of the centromere cohesion protector Shugoshin in mammals and yeast. Immunostaining results indicate that phosphorylated H2A-Thr133 signals bridged those of the centromeric H3 histone variant CENH3 by using a plant displaying yellow fluorescent protein-CENH3 signals and H2A-Thr133 is phosphorylated in different cell types. During mitosis, H2A-Thr133 phosphorylation becomes strong in metaphase and is specific to centromere regions but drops during later anaphase and telophase. Immunostaining for several maize dicentric chromosomes revealed that the inactive centromeres have lost phosphorylation of H2A-Thr133. During meiosis in maize meiocytes, H2A phosphorylation becomes strong in the early pachytene stage and increases to a maximum at metaphase I. In the maize meiotic mutant afd1 (absence of first division), sister chromatids show equational separation at metaphase I, but there are no changes in H2A-Thr-133 phosphorylation during meiosis compared with the wild type. In sgo1 mutants, sister chromatids segregate randomly during meiosis II, and phosphorylation of H2A-Thr-133 is observed on the centromere regions during meiosis II. The availability of such mutants in maize that lack sister cohesion and Shugoshin indicate that the signals for phosphorylation are not dependent on cohesion but on centromere activity.