Assessment of hypoxia in experimental mice tumours by [18F]fluoromisonidazole PET and pO2 electrode measurements -: Infuence of tumour volume and carbogen breathing
Assessment of hypoxia in experimental mice tumours by [18F]fluoromisonidazole PET and pO2 electrode measurements -: Infuence of tumour volume and carbogen breathing
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DOI：
10.1080/02841860260088863
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发表时间：
2002-05-01
期刊：
影响因子：
3.1
通讯作者：
Overgaard, J
中科院分区：
文献类型：
--
作者：
Bentzen, L;Keiding, S;Overgaard, J
本研究的目的是在移植到CDF 1小鼠背部的150-1500 mm（3）C3 H乳腺癌中，比较非侵入性F-18-氟咪唑（[F-18] FMISO）PET评估肿瘤缺氧与侵入性Eppendorf pO（2）测量。荷瘤小鼠在两次检查期间呼吸碳源气体（95%氧气，5%CO_2）或正常空气。在类似治疗的单独肿瘤中进行额外的放射自显影。PET [F-18] FMISO检查可显著区分卡波姆和空气呼吸小鼠的肿瘤。对于pO（2）测量，与呼吸空气的小鼠相比，碳源治疗小鼠的pO（2）测量值低于2.5 mmHg的百分比明显较低。然而，未观察到方法之间的直接相关性。对于呼吸正常空气的动物，发现肿瘤体积和Eppendorf估计的肿瘤缺氧之间存在相关性，但PET终点和肿瘤体积之间没有发现相关性。这可能是由于在坏死组织中获得的pO（2）测量值较低。放射自显影证实，与呼吸空气的动物相比，呼吸碳源的动物的肿瘤中[F-18] FMISO摄取较低，并证明了与较大肿瘤相比，小肿瘤中示踪剂摄取的异质性。
The aim of this study was to compare a non-invasive F-18-fluoromisonidazole ([F-18] FMISO) PET assessment of tumour hypoxia with invasive Eppendorf pO(2) measurements in 150-1500 mm(3) C3H mammary carcinomas transplanted on the back of CDF1 mice. The tumour-bearing mice breathed either carbogen gas (95% oxygen, 5% CO2) or normal air during bot h examinations. Additional autoradiography was performed in separate tumours treated similarly. The PET [F-18] FMISO examination significantly discriminated between tumours of carbogen and air-breathing mice. For the pO(2) measurements, there was a significantly lower percentage of measurements below 2.5 mmHg for carbogen-treated mice compared with air-breathing mice. However, no direct correlation between the methods was seen. A correlation was found between tumour volume and Eppendorf estimates of tumour hypoxia for the animals breathing normal air, but no correlation was found between the PET endpoint and tumour volume. This may be due to low pO(2) measurements obtained in necrotic tissue. Autoradiography confirmed lower [F-18] FMISO uptake in tumours of carbogen-breathing animals compared with air-breathing animals, and demonstrated the heterogeneity of the tracer uptake in small compared with larger tumours.